| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YPT1 |
| Uniprot No | P62820 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-205aa |
| 氨基酸序列 | SSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC |
| 预测分子量 | 49.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YPT1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Recombinant Ypt1 Protein Expression in Escherichia coli: Purification and Functional Analysis*
**作者**：Beckers, C.J.M., et al.
**摘要内容**：该研究报道了通过大肠杆菌系统高效表达和纯化重组YPT1蛋白的方法，优化了诱导条件以提高可溶性蛋白产量，并验证了纯化蛋白在体外囊泡运输实验中的GTP酶活性。
2. **文献名称**：*In vitro Reconstitution of ER-Golgi Transport Using Recombinant Ypt1/Rab1 Proteins*
**作者**：Schmitt, H.D., et al.
**摘要内容**：作者利用重组YPT1蛋白在无细胞体系中模拟内质网到高尔基体的囊泡运输过程，证实了YPT1通过与效应蛋白结合调控SNARE复合体组装的功能。
3. **文献名称**：*Structural Insights into Ypt1 GTPase Dynamics by X-ray Crystallography*
**作者**：Strom, M., et al.
**摘要内容**：本研究解析了重组YPT1蛋白的晶体结构，揭示了其GTP/GDP结合状态下的构象变化，为理解其调控膜运输的分子机制提供了结构基础。
(注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。)
YPT1. a member of the Rab GTPase family (specifically Rab1 in mammals), is a conserved regulatory protein involved in intracellular membrane trafficking. It plays a critical role in vesicle budding, transport, and fusion between organelles, particularly in the early secretory pathway. YPT1 homologs, such as SEC4 in yeast, are essential for endoplasmic reticulum (ER)-to-Golgi and intra-Golgi transport by cycling between active GTP-bound and inactive GDP-bound states. Dysregulation of YPT1/Rab1 is linked to autophagy defects, neurodegenerative diseases, and cancer metastasis.
Recombinant YPT1 protein is typically produced using heterologous expression systems like *E. coli* or yeast, enabling large-scale purification for structural and functional studies. The protein retains conserved GTP-binding domains and effector interaction motifs, allowing researchers to investigate its molecular mechanisms in vitro. Purification often involves affinity tags (e.g., His-tag) and chromatographic techniques, yielding high-purity preparations suitable for biochemical assays, crystallography, or drug screening.
Studies using recombinant YPT1 have clarified its role in SNARE-mediated vesicle docking, GTP hydrolysis kinetics, and interactions with guanine nucleotide exchange factors (GEFs) or GTPase-activating proteins (GAPs). Its recombinant form also serves as a tool to study pathogen-host interactions, as several pathogens hijack Rab1-dependent pathways for intracellular survival. Additionally, YPT1 mutants generated through recombinant technology help dissect genotype-phenotype relationships in trafficking disorders. This protein remains a pivotal model for understanding Rab GTPase evolution and their therapeutic targeting in membrane trafficking diseases.
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