| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Mcpt2 |
| Uniprot No | P15119 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-244aa |
| 氨基酸序列 | IIGGVEAKPH SRPYMAYLKF TTKNGSKERC GGFLIAPQFV MTAAHCNGSE ISVILGAHNI NKNEPTQQII KTEKTFVHPK FQYLSGFYDI MLLKLQKKAE LNSDVDVISL PSSSDFIKPG KMCWTAGWGK TGKNNPLSVT LREVELRIMD QEACKDHSDY DYQLQVCAGS PTTSKSIGQG DSGGPLVCDS VAHGIASSYE AKAPAVFTRI SYYLPWIYKV LKSK |
| 预测分子量 | 26,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCPT2重组蛋白的参考文献示例(注：以下内容基于真实研究领域整理，具体文献标题和作者为示例性描述)：
---
**1. 标题**：Recombinant mast cell protease 2 (MCPT2) expression and its role in parasite immunity
**作者**：Knight, P.A., Wright, S.H., Brown, J.K.
**摘要**：本研究通过在大肠杆菌系统中重组表达MCPT2蛋白，并优化纯化流程以获得高活性蛋白酶。实验发现，MCPT2在体外能特异性降解肠道寄生虫的体表蛋白，提示其可能在宿主抗寄生虫免疫防御中发挥关键作用。
---
**2. 标题**：Structural characterization of recombinant mouse mast cell protease 2
**作者**：Stevens, R.L., McNeil, H.P., Friend, D.S.
**摘要**：文章解析了重组小鼠MCPT2的晶体结构，揭示了其底物结合口袋的独特构象。通过酶动力学分析，发现MCPT2对特定肽底物的水解活性受钙离子调控，为开发靶向抑制剂提供了结构基础。
---
**3. 标题**：Functional analysis of MCPT2 in mucosal inflammation using recombinant protein
**作者**：Miller, H.R., Pemberton, A.D., Huntley, J.F.
**摘要**：利用重组MCPT2蛋白，研究其在肠道黏膜炎症模型中的作用。结果表明，MCPT2通过降解紧密连接蛋白破坏上皮屏障功能，加剧炎症反应，提示其作为炎症性疾病治疗靶点的潜力。
---
**4. 标题**：Enzymatic properties of recombinant MCPT2 in allergic asthma models
**作者**：Thakker, M., Hellman, L., Huang, C.
**摘要**：本研究通过杆状病毒系统表达重组MCPT2.并验证其与过敏原的相互作用。动物实验表明，MCPT2缺失可减少气道高反应性，而外源性重组蛋白加剧炎症，证明其参与哮喘病理过程。
---
(注：以上文献信息为示例，实际引用时需以具体检索结果为准。)
**Background of Recombinant Mcpt2 Protein**
Mast cell protease 2 (Mcpt2) is a chymase enzyme predominantly expressed in mucosal mast cells, playing a critical role in immune responses, particularly in host defense against parasitic infections and modulation of inflammatory processes. As a member of the serine protease family, Mcpt2 is characterized by its chymotryptic substrate specificity, cleaving proteins after aromatic residues. It is stored in mast cell granules and released upon activation, contributing to tissue remodeling, cytokine activation, and pathogen clearance.
Recombinant Mcpt2 protein is generated through genetic engineering, typically using prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., insect or mammalian cell) expression systems. This approach allows large-scale production of the protein with high purity and consistency, overcoming limitations of isolating native Mcpt2 from biological tissues. The recombinant form retains enzymatic activity and structural integrity, enabling functional studies on its interactions with substrates, inhibitors, and signaling pathways.
Research on Mcpt2 has focused on its dual role in health and disease. While it aids in combating helminth infections and regulating inflammation, dysregulated Mcpt2 activity is linked to pathological conditions such as fibrosis, asthma, and cardiovascular diseases. Recombinant Mcpt2 is instrumental in dissecting these mechanisms, screening therapeutic inhibitors, and developing mast cell-targeted therapies. Additionally, it serves as a tool to study mast cell biology, including granule maturation and protease networks.
The development of recombinant Mcpt2 has advanced translational studies, bridging gaps between *in vitro* models and *in vivo* applications. Challenges remain in mimicking post-translational modifications and ensuring functional equivalence to native proteins, but ongoing optimization of expression systems continues to enhance its utility in biomedical research.
×
