| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | tsaE |
| Uniprot No | P0AF67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG |
| 预测分子量 | 16.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与tsaE重组蛋白相关的参考文献，按文献名称、作者和摘要内容简要概括：
1. **文献名称**: "Functional characterization of the TsaE protein in the bacterial antioxidant system"
**作者**: Smith A, et al.
**摘要**: 本研究在大肠杆菌中重组表达了TsaE蛋白，并验证其作为TsaEFBD复合物的ATP酶亚基功能。通过体外实验证明重组TsaE与TsaB/TsaD形成复合物，参与硫代硫酸盐代谢和氧化应激保护。
2. **文献名称**: "Structural insights into the TsaE-TsaD complex involved in tRNA modification"
**作者**: Johnson R, et al.
**摘要**: 通过X射线晶体学解析了重组表达的TsaE与TsaD的复合物结构，揭示了二者在tRNA硫修饰通路中的相互作用机制，为细菌中tRNA稳定性调控提供了分子基础。
3. **文献名称**: "Recombinant production and biochemical analysis of the TsaE protein from Thermus thermophilus"
**作者**: Müller C, et al.
**摘要**: 报道了嗜热菌TsaE重组蛋白的高温稳定性表达与纯化方法，并证实其在65°C下仍保持ATP水解活性，为极端环境下抗氧化系统的研究提供工具。
注：上述文献信息为示例性概括，实际引用时建议通过PubMed或Web of Science以"tsaE recombinant protein"为关键词检索最新文献。
**Background of TsaE Recombinant Protein**
TsaE is a conserved bacterial protein belonging to the TsaE/TsaD family, which plays a critical role in the thiol modification and quality control of transfer RNAs (tRNAs). It is a component of the TsaABCDE system, initially identified in *Escherichia coli* as part of a stress-responsive pathway involved in detoxifying reactive oxygen species (ROS). The TsaE protein, encoded by the *tsaE* gene, functions as an ATPase, providing energy for the thiolation of specific tRNAs—a post-transcriptional modification essential for maintaining translational accuracy under oxidative stress.
Structurally, TsaE adopts an ATP-binding domain and interacts with other components of the Tsa system, particularly TsaD, to form a multi-enzyme complex. This complex ensures the proper maturation of tRNAs by incorporating sulfur groups at wobble uridine residues, thereby enhancing their stability and decoding efficiency. Dysregulation of tRNA thiolation has been linked to growth defects and increased sensitivity to oxidative damage, highlighting TsaE's role in bacterial stress adaptation.
Recombinant TsaE protein is produced using heterologous expression systems (e.g., *E. coli*) for biochemical and structural studies. Its recombinant form enables detailed analysis of ATPase activity, protein-protein interactions, and mechanistic insights into tRNA modification pathways. Additionally, TsaE homologs are found in diverse bacteria, including pathogens, making it a potential target for antimicrobial strategies.
Recent studies also explore TsaE's involvement in human pathogens like *Pseudomonas aeruginosa*, where it contributes to virulence and oxidative stress resistance. Understanding TsaE's function could inform therapeutic approaches targeting bacterial stress response systems. Overall, TsaE recombinant protein serves as a vital tool for dissecting fundamental mechanisms of tRNA biology and microbial stress resilience.
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