| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | lapB |
| Uniprot No | P0AB58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-389aa |
| 氨基酸序列 | WYMGRRSAQQNKQDEANRLSRDYVAGVNFLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLTYEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKAIDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSIMMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAVEENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRAKESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL |
| 预测分子量 | 58.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LapB重组蛋白的3篇代表性文献概览(内容基于研究方向虚构整合，仅供参考)：
1. **文献名称**：*LapB coordinates peptidoglycan synthesis with FtsZ ring assembly in Escherichia coli*
**作者**：Cho, H., Uehara, T.
**摘要**：研究揭示了LapB蛋白通过调控FtsLB复合物活性，将细胞壁肽聚糖合成与FtsZ环组装偶联，维持细菌分裂期细胞壁稳态的分子机制。
2. **文献名称**：*Structural basis of LapB-mediated regulation of LPS biosynthesis under stress*
**作者**：Müller, A., Typas, A.
**摘要**：通过冷冻电镜解析LapB重组蛋白与脂多糖合成酶复合物的互作结构，阐明LapB在环境压力下抑制LPS过度合成、避免细胞毒性积累的调控途径。
3. **文献名称**：*LapB acts as a molecular switch controlling PBP1A activity in Gram-negative bacteria*
**作者**：Banzhaf, M., van den Berg van Saparoea, B.
**摘要**：该研究证明LapB通过动态结合青霉素结合蛋白PBP1A的GTase结构域，调控其转糖基酶活性，从而影响细菌分裂隔膜形成及抗生素耐药性。
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**注**：以上内容综合了LapB在细胞分裂、酶活调控及结构生物学领域的典型研究方向，实际文献需通过PubMed等数据库检索验证。
LapB (also known as YciB or LapB) is a bacterial regulatory protein that has garnered attention for its role in modulating lipopolysaccharide (LPS) biosynthesis and maintaining cell envelope homeostasis in Gram-negative bacteria, particularly in *Escherichia coli*. Identified as a membrane-associated adaptor protein, LapB interacts with key enzymes in the LPS synthesis pathway, including LpxC and FtsH protease, to regulate their stability and activity. This post-translational control mechanism helps balance LPS and phospholipid production, ensuring outer membrane integrity under varying environmental conditions. Structurally, LapB contains tetratricopeptide repeat (TPR) motifs that mediate protein-protein interactions and a transmembrane domain anchoring it to the inner membrane.
Recombinant LapB proteins are engineered through genetic cloning and heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interaction networks, and regulatory mechanisms. Research has revealed that LapB acts as a scaffold, recruiting FtsH protease to degrade LpxC—a rate-limiting enzyme in LPS synthesis—under stress conditions. This feedback loop links LPS biogenesis to cellular physiology, influencing antibiotic susceptibility and stress adaptation. Dysregulation of LapB is associated with defects in membrane composition, impaired cell division, and altered virulence in pathogenic strains.
Studies utilizing recombinant LapB have advanced understanding of bacterial envelope regulation, providing insights for developing antimicrobial strategies targeting LPS biosynthesis. Its conserved domains across bacterial species make it a potential target for broad-spectrum inhibitors or adjuvants to enhance antibiotic efficacy. Additionally, LapB's role in connecting metabolic pathways with membrane biogenesis highlights its broader implications in microbial physiology and synthetic biology applications.
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