| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Mcpt1 |
| Uniprot No | P11034 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-246aa |
| 氨基酸序列 | IIGGVEARPH SRPYMAHLKI ITDRGSEDRC GGFLIAPQFV LTAAHCKGRE ITVTLGAHDV SKSESTQQRI KVEKQIIHKN YNVSFNLYDI MLLKLEEKAE LTPTVDVIPL PGPSDFIDPG KMCWTAGWGK TGEKEPTSET LREVELRIMD KEACKMYKHY DYNFQVCVGS STKLKTAYMG DSGGPLLCAG VAHGIVSYGD SHGKPPAVFT RISAYVPWIK TVINGK |
| 预测分子量 | 27,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您模拟的3篇关于Mcpt1重组蛋白的参考文献示例(请注意这些为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*Expression and Purification of Recombinant Mcpt1 in Escherichia coli for Protease Activity Analysis*
**作者**：Zhang L., et al.
**摘要**：本研究成功构建了Mcpt1基因的大肠杆菌表达系统，通过His标签亲和层析纯化获得高纯度重组蛋白。酶动力学实验证实重组Mcpt1具有胰蛋白酶样活性，最适pH为8.0.可特异性切割合成底物Tosyl-GPR-pNA。
2. **文献名称**：*Crystal Structure of Mcpt1 Reveals Substrate-binding Specificity*
**作者**：Tanaka K., et al.
**摘要**：作者在昆虫细胞中表达Mcpt1重组蛋白并通过X射线晶体学解析其三维结构(分辨率2.1Å)。结构分析显示其底物结合口袋的带电残基分布与已知肥大细胞蛋白酶差异显著，解释了其对精氨酸/赖氨酸残基的强选择性。
3. **文献名称**：*Mcpt1 Recombinant Protein Induces Mast Cell-Mediated Allergic Responses in Murine Models*
**作者**：Chen H., et al.
**摘要**：通过哺乳动物细胞表达系统制备重组Mcpt1.动物实验显示其能直接激活肥大细胞释放组胺和IL-4.且该效应可被丝氨酸蛋白酶抑制剂AEBSF阻断，提示Mcpt1可能通过PAR-2受体途径参与过敏反应调控。
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**建议检索真实文献的关键词**：
- "MCPT1 recombinant protein expression"
- "Mast cell protease 1 structure/function"
- "Mcpt1 protease activity assay"
**推荐数据库**：PubMed、Google Scholar、ScienceDirect(可使用上述关键词筛选近5年文献)
Mast cell protease 1 (MCPT-1), also known as chymase, is a serine protease predominantly expressed in mast cells, playing critical roles in inflammatory responses and tissue remodeling. As a key mediator in mast cell granules, MCPT-1 is released upon mast cell activation and participates in diverse physiological and pathological processes, including allergic reactions, wound healing, and cardiovascular diseases. Its enzymatic activity targets extracellular matrix components (e.g., collagen) and bioactive peptides (e.g., angiotensin I), influencing vascular tone and fibrosis pathways.
Recombinant MCPT-1 protein is engineered to study its structure-function relationships and therapeutic potential. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant form retains enzymatic activity while enabling controlled experimental conditions. Its 238-amino-acid structure includes a conserved catalytic triad (His, Asp, Ser) and substrate-binding pockets critical for specificity. Researchers utilize recombinant MCPT-1 to investigate mast cell biology, screen protease inhibitors, and explore its dual role in tissue repair versus pathological remodeling. Current studies focus on its involvement in chronic inflammatory diseases, such as asthma and atherosclerosis, where dysregulated MCPT-1 activity exacerbates tissue damage. The development of recombinant variants (e.g., catalytically inactive mutants) further aids in dissecting its non-enzymatic signaling functions. As mast cell-targeted therapies gain momentum, recombinant MCPT-1 serves as a vital tool for drug discovery and mechanistic studies.
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