| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | SES1 |
| Uniprot No | P07284 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MLDINQFIEDKGGNPELIRQSQKARNASVEIVDEIISDYKDWVKTRFELDELNKKFNKLQKDIGLKFKNKEDASGLLAEKEKLTQQKKELTEKEQQEDKDLKKKVFQVGNIVHPSVVVSNDEENNELVRTWKPEDLEAVGPIASVTGKPASLSHHEILLRLDGYDPDRGVKICGHRGYFFRNYGVFLNQALINYGLQFLAAKGYIPLQAPVMMNKELMSKTAQLSEFDEELYKVIDGEDEKYLIATSEQPISAYHSGEWFEKPQEQLPIHYVGYSSCFRREAGSHGKDAWGVFRVHAFEKIEQFVITEPEKSWEEFEKMISYSEEFYKSLKLPYRIVGIVSGELNNAAAKKYDLEAWFPYQKEYKELVSCSNCTDYQSRNLEIRCGIKKMGDREKKYVHCLNSTLAATQRALCCILENYQTEDGLVVPEVLRKYIPGEPEFLPFVNELPKNSTSSKDKKKKN |
| 预测分子量 | 55.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SES1重组蛋白的虚构参考文献示例(仅供参考，实际文献需通过学术数据库检索)：
1. **文献名称**: "Cloning and Expression of SES1 Recombinant Protein in E. coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过大肠杆菌系统高效表达SES1重组蛋白的优化策略，验证了该蛋白的分子伴侣活性及在体外促进靶蛋白折叠的功能。
2. **文献名称**: "SES1 as a Stress-Responsive Protein in Cancer Cell Survival"
**作者**: Tanaka K, et al.
**摘要**: 探讨了重组SES1蛋白在肿瘤细胞应激反应中的作用，证明其通过抑制内质网应激介导的凋亡通路增强癌细胞存活。
3. **文献名称**: "Structural Characterization of Recombinant SES1 by Cryo-EM"
**作者**: Wang Y, et al.
**摘要**: 利用冷冻电镜解析了SES1重组蛋白的三维结构，揭示了其与热休克蛋白HSP70相互作用的分子机制。
4. **文献名称**: "Therapeutic Potential of SES1 in Neurodegenerative Diseases"
**作者**: Smith J, et al.
**摘要**: 在小鼠模型中验证了重组SES1蛋白通过减少错误蛋白聚集改善阿尔茨海默病病理表型的潜力。
**注意**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台以“SES1 recombinant protein”或相关关键词检索。若需具体文献，请提供更多背景信息(如SES1的全称或研究领域)。
**Background of SES1 Recombinant Protein**
SES1 (Selenoprotein S, SelS), also known as VIMP (VCP-interacting membrane protein), is a ubiquitously expressed endoplasmic reticulum (ER)-resident protein involved in critical cellular processes, including ER stress response, redox homeostasis, and inflammation regulation. It belongs to the selenoprotein family, characterized by the incorporation of selenocysteine (Sec) at its active site, which is encoded by a UGA codon—a rare feature requiring specific translational machinery. SES1 plays a pivotal role in the ER-associated degradation (ERAD) pathway by interacting with valosin-containing protein (VCP/p97), facilitating the clearance of misfolded proteins and maintaining ER proteostasis. This function links SES1 to cellular stress adaptation and survival mechanisms.
The recombinant form of SES1 is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to enable detailed biochemical and functional studies. Recombinant SES1 retains its structural and functional integrity, allowing researchers to explore its antioxidant properties, anti-inflammatory effects, and regulatory roles in metabolic diseases. For instance, SES1 is implicated in modulating insulin resistance, atherosclerosis, and neurodegenerative disorders, making it a potential therapeutic target. Its recombinant production has facilitated investigations into its molecular interactions, post-translational modifications, and mechanisms underlying disease pathologies.
Studies highlight SES1's dual role in health and disease: while it protects against oxidative stress and inflammation, dysregulation of SES1 expression correlates with chronic inflammatory conditions and metabolic syndromes. Recombinant SES1 thus serves as a vital tool for deciphering its pathophysiological relevance and advancing therapeutic strategies targeting ER stress-related diseases.
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