| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | tar |
| Uniprot No | P07017 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 212-553aa |
| 氨基酸序列 | IRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF |
| 预测分子量 | 52.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAR重组蛋白的3篇代表性文献示例(注：文献为虚构示例，仅用于展示格式)：
1. **文献名称**：Structural Insights into HIV-1 TAR RNA Interaction with Recombinant Tat Protein
**作者**：Smith A, et al.
**摘要**：该研究通过重组表达HIV-1 Tat蛋白，结合X射线晶体学技术解析了Tat与TAR RNA复合物的三维结构，揭示了关键结合位点的分子机制，为抗病毒药物设计提供了结构基础。
2. **文献名称**：High-Yield Production of Recombinant TAR-Binding Protein TRBP for Functional Studies
**作者**：Lee B, et al.
**摘要**：报道了一种高效重组表达纯化TAR RNA结合蛋白(TRBP)的方法，并通过体外实验验证其增强HIV-1病毒复制的能力，为研究宿主因子在病毒感染中的作用提供了工具。
3. **文献名称**：Engineering a Fluorescent Recombinant TAR-Tat Reporter System for Viral Transcription Monitoring
**作者**：Zhang C, et al.
**摘要**：开发了一种基于重组Tat蛋白和荧光标记TAR RNA的动态报告系统，实时监测HIV-1转录激活过程，为高通量抗病毒化合物筛选提供了新平台。
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**说明**：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索关键词如“TAR recombinant protein”、“Tat-TAR interaction”获取最新进展。经典研究可参考：
- Frankel AD, et al. (1988) *Cell* 对Tat-TAR互作的早期机制研究。
- Karn J, et al. (1999) *J Mol Biol* 关于TAR RNA结构与功能的综述。
**Background of TAR Recombinant Protein**
The TAR (Trans-Activation Response) element is a critical RNA regulatory region found in the long terminal repeat (LTR) of HIV-1. It plays a central role in viral transcription by interacting with the viral Tat (Trans-Activator of Transcription) protein and host cellular factors. The TAR RNA forms a stem-loop structure that binds Tat, recruiting cyclin-dependent kinase 9 (CDK9) and other components of the positive transcription elongation factor (P-TEFb) to enhance RNA polymerase II processivity. This interaction is essential for efficient viral replication, making TAR a key target for antiretroviral research.
Recombinant TAR proteins are engineered to study these molecular interactions in vitro. They are typically produced by cloning the TAR DNA sequence into expression vectors, followed by purification using affinity chromatography. These proteins enable detailed structural and functional analyses, such as elucidating Tat-TAR binding mechanisms or screening small-molecule inhibitors that disrupt this interaction.
Beyond HIV research, TAR recombinant proteins have applications in understanding RNA-protein dynamics, gene regulation, and nucleic acid aptamer development. Challenges include maintaining the RNA’s native conformation during production and ensuring specificity in binding assays. Recent advancements in structural biology (e.g., cryo-EM, NMR) and gene-editing tools (e.g., CRISPR) have further leveraged TAR recombinant proteins to explore novel therapeutic strategies, including gene silencing and RNA-targeted antivirals.
Overall, TAR recombinant proteins serve as vital tools for dissecting viral pathogenesis and advancing targeted therapies against HIV and related retroviruses.
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