| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | sacC |
| Uniprot No | P05656 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-677aa |
| 氨基酸序列 | ADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGHAVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKKWVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDIILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQGRSDGDSFILSSASGSDFTYESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLVKFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIYLDDRLVIDAHDSVFSEGQFGLNVWDATAVFQNVTKES |
| 预测分子量 | 77.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and expression of the sacC gene encoding levansucrase from Bacillus subtilis in Escherichia coli"**
*Author: Degrassi G., et al.*
摘要：该研究成功将枯草芽孢杆菌的sacC基因克隆至大肠杆菌中表达，重组酶表现出果聚糖蔗糖酶活性，并分析了其酶学特性及底物特异性。
2. **"Enhanced production of recombinant SacC levansucrase by optimization of induction conditions in Pichia pastoris"**
*Author: Li Y., et al.*
摘要：通过优化毕赤酵母表达系统中的诱导条件(温度、pH、甲醇浓度)，显著提高了sacC重组蛋白的产量，并验证了其合成低聚果糖的应用潜力。
3. **"Structural and functional analysis of SacC levansucrase reveals key residues for polymer synthesis"**
*Author: Martínez-Fleites C., et al.*
摘要：通过X射线晶体学解析sacC重组蛋白的三维结构，鉴定出影响果聚糖链延伸的关键活性位点残基，为酶工程改造提供理论依据。
4. **"High-level extracellular production of recombinant SacC in Bacillus licheniformis for industrial levan production"**
*Author: Wang H., et al.*
摘要：利用地衣芽孢杆菌表达系统实现sacC重组蛋白的高效分泌表达，优化发酵工艺后酶活达到120 U/mL，适用于大规模生产果聚糖生物材料。
The sacC gene, originally identified in *Bacillus subtilis*, encodes levansucrase (SacC), an enzyme that catalyzes the synthesis of fructan polymers (levans) from sucrose. Levansucrase plays a dual role: it hydrolyzes sucrose to release glucose and fructose while polymerizing fructose residues into β-2.6-linked levan, a polysaccharide with applications in food, pharmaceutical, and biotechnology industries. Recombinant SacC protein production emerged to overcome limitations in native enzyme yield and purity. By cloning the sacC gene into heterologous expression systems like *E. coli* or yeast, researchers achieve scalable, high-efficiency production of the enzyme with simplified purification via affinity tags (e.g., His-tag). Recombinant SacC retains catalytic activity and offers flexibility for protein engineering to enhance stability, substrate specificity, or thermotolerance. Its industrial relevance lies in synthesizing prebiotic fructooligosaccharides (FOS) and levan, which are valued as dietary fibers, drug delivery matrices, or biofilms. Additionally, SacC serves as a model enzyme for studying glycosyltransferase mechanisms and protein secretion pathways. Recent advances focus on optimizing expression conditions, fusion protein designs, and immobilization techniques to improve industrial applicability. As sustainable bioprocessing gains momentum, recombinant SacC exemplifies how microbial enzymes can be harnessed for eco-friendly production of functional biomaterials.
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