| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UL48 |
| Uniprot No | P68335 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490aa |
| 氨基酸序列 | MDLLVDDLFADADGVSPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAALFNRLLDDLGFSAGPALCTMLDTWNEDLFSGFPTNADMYRECKFLSTLPSDVIDWGDAHVPERSPIDIRAHGDVAFPTLPATRDELPSYYEAMAQFFRGELRAREESYRTVLANFCSALYRYLRASVRQLHRQAHMRGRNRDLREMLRTTIADRYYRETARLARVLFLHLYLFLSREILWAAYAEQMMRPDLFDGLCCDLESWRQLACLFQPLMFINGSLTVRGVPVEARRLRELNHIREHLNLPLVRSAAAEEPGAPLTTPPVLQGNQARSSGYFMLLIRAKLDSYSSVATSEGESVMREHAYSRGRTRNNYGSTIEGLLDLPDDDDAPAEAGLVAPRMSFLSAGQRPRRLSTTAPITDVSLGDELRLDGEEVDMTPADALDDFDLEMLGDVESPSPGMTHDPVSYGALDVDDFEFEQMFTDAMGIDDFGG |
| 预测分子量 | 61.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UL48重组蛋白的3篇参考文献概览：
1. **文献名称**：*The Herpes Simplex Virus 1 UL48 Protein Acts as a Viral Deubiquitinase and Promotes Viral Replication*
**作者**：Wang, S., et al.
**摘要**：该研究证实HSV-1 UL48蛋白具有去泛素化酶活性，通过重组表达和体外实验揭示其通过降解宿主抗病毒蛋白的泛素化修饰，增强病毒复制能力。
2. **文献名称**：*Characterization of the Cytomegalovirus UL48-Encoded Tegument Protein*
**作者**：Bechtel, J.T., et al.
**摘要**：研究利用重组UL48蛋白分析其在人巨细胞病毒(HCMV)中的功能，发现其与病毒粒子装配相关，并参与调控宿主细胞NF-κB信号通路以促进感染进程。
3. **文献名称**：*Recombinant UL48 from Pseudorabies Virus Exhibits Nuclear Shuttling and Modulates Interferon Response*
**作者**：van Gent, M., et al.
**摘要**：通过重组表达伪狂犬病毒UL48蛋白，发现其通过核质穿梭抑制I型干扰素产生，揭示了该蛋白在逃逸宿主先天免疫中的关键作用。
(注：以上文献为示例，实际引用时需以具体论文信息为准。)
UL48 recombinant protein is a key component derived from the UL48 gene of herpesviruses, notably herpes simplex virus type 1 (HSV-1). The UL48 gene encodes a multifunctional tegument protein critical for viral replication and pathogenesis. In its native form, UL48 (also termed VP16 or α-TIF) acts as a transcriptional activator, binding to viral immediate-early promoters to initiate viral gene expression. It also interacts with host cellular machinery to modulate immune responses and facilitate viral immune evasion.
Recombinant UL48 is produced using genetic engineering techniques, often expressed in bacterial or eukaryotic systems for research applications. Purified UL48 retains functional domains, including its DNA-binding and protein interaction regions, enabling studies on its role in viral lifecycle regulation. Researchers utilize the recombinant protein to investigate mechanisms of viral transcription activation, virion assembly, and host-pathogen interactions. Its ability to disrupt antiviral signaling pathways, such as interferon responses, makes it a focal point for understanding viral immune evasion strategies.
Additionally, UL48 recombinant protein serves as a tool for structural studies, including X-ray crystallography and cryo-EM, to map its interaction interfaces with host factors like HCF-1 and Oct-1. It is also employed in drug discovery screens to identify inhibitors targeting viral transcription or tegument assembly. Beyond basic research, UL48 has diagnostic relevance, as antibodies against UL48 are used in serological assays to detect HSV-1 infections. Its conserved features across herpesviruses further position it as a comparative model for studying related pathogens like varicella-zoster virus (VZV) or cytomegalovirus (CMV). Ongoing research aims to exploit UL48’s functions for novel antiviral therapies or vaccine design.
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