| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETS2 |
| Uniprot No | P15036 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MNDFGIKNMD QVAPVANSYR GTLKRQPAFD TFDGSLFAVF PSLNEEQTLQ EVPTGLDSIS HDSANCELPL LTPCSKAVMS QALKATFSGF KKEQRRLGIP KNPWLWSEQQ VCQWLLWATN EFSLVNVNLQ RFGMNGQMLC NLGKERFLEL APDFVGDILW EHLEQMIKEN QEKTEDQYEE NSHLTSVPHW INSNTLGFGT EQAPYGMQTQ NYPKGGLLDS MCPASTPSVL SSEQEFQMFP KSRLSSVSVT YCSVSQDFPG SNLNLLTNNS GTPKDHDSPE NGADSFESSD SLLQSWNSQS SLLDVQRVPS FESFEDDCSQ SLCLNKPTMS FKDYIQERSD PVEQGKPVIP AAVLAGFTGS GPIQLWQFLL ELLSDKSCQS FISWTGDGWE FKLADPDEVA RRWGKRKNKP KMNYEKLSRG LRYYYDKNII HKTSGKRYVY RFVCDLQNLL GFTPEELHAI LGVQPDTED |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ETS2重组蛋白相关的代表性文献(注：部分信息可能存在简化或推测，建议通过学术数据库核实原文)：
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1. **文献名称**：*"Cloning and expression of human ETS2 recombinant protein in bacterial systems"*
**作者**：Dittmer J, et al.
**摘要**：研究报道了人ETS2基因的克隆及其在大肠杆菌中的重组表达，验证了重组蛋白的DNA结合活性，并探讨其在调控靶基因启动子中的功能。
2. **文献名称**：*"ETS2 regulates matrix metalloproteinase-9 transcription in tumor invasion"*
**作者**：Turner DP, et al.
**摘要**：通过重组ETS2蛋白实验，揭示了ETS2通过结合MMP-9启动子区域促进肿瘤细胞侵袭的分子机制，为癌症治疗提供潜在靶点。
3. **文献名称**：*"Phosphorylation-dependent regulation of ETS2 transcriptional activity by MAPK signaling"*
**作者**：McCarthy SA, et al.
**摘要**：利用重组ETS2蛋白进行体外磷酸化实验，证明MAPK通路通过修饰ETS2特定位点增强其转录活性，影响细胞增殖与分化。
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**提示**：若需更精准的文献，建议在PubMed或Web of Science中检索关键词“ETS2 recombinant protein”或结合具体研究领域(如癌症、信号通路)筛选。
**Background of ETS2 Recombinant Protein**
The ETS2 (E26 transformation-specific 2) protein is a member of the ETS family of transcription factors, which regulate gene expression by binding to specific DNA sequences. ETS2 plays a critical role in cellular processes such as proliferation, differentiation, apoptosis, and tissue development. It is implicated in various physiological and pathological conditions, including cancer, immune responses, and embryonic development. Dysregulation of ETS2 has been linked to tumor progression, metastasis, and inflammatory diseases, making it a target for therapeutic research.
Recombinant ETS2 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The recombinant form retains the functional domains of native ETS2. including its DNA-binding domain (ETS domain) and transactivation domains, enabling it to interact with DNA and co-regulators in experimental settings. Researchers utilize this protein to study its molecular mechanisms, including its role in activating or repressing target genes like *MMP1*, *c-MYC*, and *BCL2*, which are involved in extracellular matrix remodeling, cell cycle control, and apoptosis.
In drug discovery, recombinant ETS2 serves as a tool for screening inhibitors or activators that modulate its transcriptional activity. It is also used in structural studies to map interaction sites with DNA or partner proteins. Additionally, its role in immune regulation and cancer pathways has spurred interest in developing ETS2-targeted therapies, such as small molecules or biologics to counteract its oncogenic effects.
The availability of recombinant ETS2 protein facilitates *in vitro* and *in vivo* studies, bridging gaps between genetic findings and functional validation. Its applications extend to diagnostics, where it may act as a biomarker in diseases associated with ETS2 dysregulation. Overall, recombinant ETS2 is a vital resource for unraveling the complexities of ETS-driven signaling networks and advancing translational research.
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