| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | emm5 |
| Uniprot No | P02977 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-461aa |
| 氨基酸序列 | AVTRGTINDPQRAKEALDKYELENHDLKTKNEGLKTENEGLKTENEGLKTENEGLKTEKKEHEAENDKLKQQRDTLSTQKETLEREVQNTQYNNETLKIKNGDLTKELNKTRQELANKQQESKENEKALNELLEKTVKDKIAKEQENKETIGTLKKILDETVKDKIAKEQENKETIGTLKKILDETVKDKLAKEQKSKQNIGALKQELAKKDEANKISDASRKGLRRDLDASREAKKQLEAEHQKLEEQNKISEASRKGLRRDLDASREAKKQLEAEQQKLEEQNKISEASRKGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAGKASDSQTPDTKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPST |
| 预测分子量 | 52.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于emm5重组蛋白的模拟参考文献示例(仅供参考，非真实文献)：
1. **标题**：*Immunogenicity of recombinant M5 protein in a murine model of Streptococcus pyogenes infection*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌表达系统制备emm5重组蛋白，证实其在小鼠模型中可诱导保护性抗体，降低链球菌感染后的组织损伤。
2. **标题**：*Structural and functional analysis of the emm5 streptococcal surface protein*
**作者**：Lee C, et al.
**摘要**：通过X射线晶体学解析emm5重组蛋白的结构，揭示其与宿主纤维蛋白原结合的机制，为靶向药物设计提供依据。
3. **标题**：*Expression optimization and purification of emm5 recombinant protein for serodiagnosis*
**作者**：Zhang Y, et al.
**摘要**：优化emm5蛋白在原核系统中的可溶性表达条件，验证其作为抗原用于A组链球菌血清学检测的灵敏度和特异性。
4. **标题**：*Cross-reactivity evaluation of emm5-based vaccines against divergent Streptococcus strains*
**作者**：Johnson R, et al.
**摘要**：评估基于emm5重组蛋白的疫苗对多种链球菌血清型的交叉保护效果，发现其对部分亚型具有广谱免疫应答潜力。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词：*emm5 recombinant protein, Streptococcus pyogenes M protein, M5 vaccine*。
EMM5 (Extracellular Matrix Metalloproteinase Inducer 5), also known as basigin (BSG) or CD147. is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays a pivotal role in regulating extracellular matrix (ECM) remodeling by inducing the expression of matrix metalloproteinases (MMPs), particularly in stromal and tumor cells. EMM5 is widely expressed in various tissues and is implicated in physiological processes such as tissue repair, immune response, and embryonic development. However, its dysregulation has been linked to pathological conditions, including cancer progression, inflammatory diseases, and viral infections. In cancer, EMM5 facilitates tumor invasion, angiogenesis, and metastasis by promoting MMP-mediated ECM degradation and modulating cell-cell interactions.
Recombinant EMM5 protein is engineered for research and therapeutic applications. It is typically produced using expression systems like Escherichia coli or mammalian cells, ensuring proper post-translational modifications for functional studies. The recombinant form often includes tags (e.g., His-tag) for purification and detection. Researchers utilize EMM5 recombinant protein to study its interactions with integrins, cyclophilins, and MMPs, shedding light on signaling pathways involved in disease mechanisms. Additionally, it serves as a tool for developing inhibitors targeting EMM5-MMP axes in anticancer therapies. Despite its promise, challenges remain in optimizing recombinant EMM5 stability and activity for clinical translation. Ongoing studies aim to clarify its structural motifs and binding partners, further unlocking its potential as a diagnostic or therapeutic target.
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