| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HLA-DQB1 |
| Uniprot No | P01920 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-227aa |
| 氨基酸序列 | RDSPEDFVYQFKAMCYFTNGTERVRYVTRYIYNREEYARFDSDVEVYRAVTPLGPPDAEYWNSQKEVLERTRAELDTVCRHNYQLELRTTLQRRVEPTVTISPSRTEALNHHNLLVCSVTDFYPAQIKVRWFRNDQEETTGVVSTPLIRNGDWTFQILVMLEMTPQHGDVYTCHVEHPSLQNPITVEWRAQSESA |
| 预测分子量 | 27.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HLA-DQB1重组蛋白的模拟参考文献示例(注：文献为虚构，仅用于示例)：
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1. **文献名称**：*Expression and Structural Characterization of Recombinant HLA-DQB1 in a Mammalian Cell System*
**作者**：Smith A, et al.
**摘要**：本研究利用哺乳动物表达系统成功表达了HLA-DQB1*05:01重组蛋白，通过X射线晶体学解析其三维结构，并验证其与抗原多肽的结合能力，为研究II类HLA分子的构效关系提供了基础。
2. **文献名称**：*HLA-DQB1 Recombinant Protein Binding to Gliadin Peptides in Celiac Disease Pathogenesis*
**作者**：Jones B, et al.
**摘要**：通过体外实验证实，重组HLA-DQB1*02:01蛋白可特异性结合谷蛋白多肽，揭示其在乳糜泻患者中触发T细胞免疫反应的分子机制，为疾病诊断标记物的开发提供依据。
3. **文献名称**：*Development of an Autoantibody Detection Assay Using Recombinant HLA-DQB1 for Type 1 Diabetes Screening*
**作者**：Chen L, et al.
**摘要**：研究团队基于大肠杆菌表达的重组HLA-DQB1*03:02蛋白，建立了一种高灵敏度的ELISA检测方法，用于筛查1型糖尿病患者的自身抗体，显著提高了早期诊断效率。
4. **文献名称**：*Functional Analysis of HLA-DQB1 Allelic Variants in Autoimmune Encephalomyelitis*
**作者**：Wang Y, et al.
**摘要**：通过比较不同HLA-DQB1等位基因(如*06:02和*05:03)重组蛋白的抗原呈递功能，发现特定变异体在实验性自身免疫性脑脊髓炎模型中显著增强致病性T细胞活化，提示其与多发性硬化的潜在关联。
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以上文献示例聚焦于重组蛋白的表达、结构解析、疾病机制及诊断应用，涵盖基础研究与临床转化方向。实际研究中建议通过学术数据库(如PubMed、Web of Science)检索具体文献。
**Background of HLA-DQB1 Recombinant Protein**
The HLA-DQB1 gene encodes a subunit of the HLA-DQ heterodimer, a key component of the major histocompatibility complex (MHC) class II molecules. These molecules are primarily expressed on antigen-presenting cells (e.g., dendritic cells, B cells) and play a central role in adaptive immunity by presenting exogenous peptide antigens to CD4+ T cells. HLA-DQB1 exhibits high polymorphism, with numerous allelic variants linked to autoimmune diseases, including type 1 diabetes, celiac disease, and multiple sclerosis.
Recombinant HLA-DQB1 proteins are engineered to study its structure-function relationships, antigen-binding specificity, and disease mechanisms. They are typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper folding, glycosylation, and dimerization with partner subunits like HLA-DQA1. Purification often involves affinity chromatography followed by biochemical validation (e.g., SDS-PAGE, Western blot).
These proteins are vital tools for investigating HLA-peptide interactions, T cell receptor recognition, and autoimmune pathogenesis. For example, specific HLA-DQB1 variants (e.g., DQB1*03:02 in celiac disease) can be expressed recombinantly to analyze their binding to gluten-derived peptides, aiding in diagnostic or therapeutic development. Additionally, recombinant HLA-DQ molecules are used in epitope mapping, vaccine design, and evaluating immune responses in transplantation.
Challenges include maintaining conformational stability, mimicking post-translational modifications, and addressing allelic diversity. Advances in structural biology (e.g., cryo-EM) and recombinant technology continue to enhance their application in immunology research and precision medicine.
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