| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ins1 |
| Uniprot No | P01325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-108aa |
| 氨基酸序列 | FVKQHLCGPHLVEALYLVCGERGFFYTPKSRREVEDPQVEQLELGGSPGDLQTLALEVARQKRGIVDQCCTSICSLYQLENYCN |
| 预测分子量 | 16.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ins1重组蛋白的3篇参考文献示例(内容为虚构，仅用于格式参考)：
1. **《高效表达Ins1重组蛋白的大肠杆菌系统优化研究》**
作者：Zhang L, et al.
摘要：本研究通过优化大肠杆菌表达条件(如诱导温度、IPTG浓度)，显著提升了Ins1重组蛋白的产量，并验证其与天然胰岛素类似的生物活性。
2. **《基于酵母系统的Ins1重组蛋白分泌表达及纯化》**
作者：Wang Y, et al.
摘要：开发了一种利用毕赤酵母分泌表达Ins1重组蛋白的方法，通过两步层析纯化获得高纯度产物，为规模化生产提供了新策略。
3. **《重组Ins1蛋白在糖尿病小鼠模型中的降糖效果评估》**
作者：Chen H, et al.
摘要：实验证明，通过注射重组Ins1蛋白可有效降低链脲佐菌素诱导的糖尿病小鼠血糖水平，且无显著免疫原性反应，具有潜在治疗价值。
Insulin-1 (Ins1) is a peptide hormone primarily produced by pancreatic β-cells in rodents, playing a critical role in glucose homeostasis. Unlike humans, who express a single insulin gene, mice and rats possess two non-allelic insulin genes, *Ins1* and *Ins2*, both encoding functional insulin proteins. Ins1 contributes to ~20-30% of pancreatic insulin in mice, while Ins2 dominates in rats. Though structurally and functionally similar, their expression patterns differ: Ins1 is β-cell-specific, whereas Ins2 is also expressed in brain and yolk sac tissues.
Recombinant Ins1 protein is engineered using biotechnological methods, typically involving the insertion of the *Ins1* gene into expression systems like *E. coli* or yeast. This allows large-scale production of pure, bioactive Ins1 for research. The recombinant form retains the native protein’s structure—comprising A and B chains linked by disulfide bonds—and biological activity, enabling precise studies without batch variability from animal-derived insulin.
Ins1 recombinant protein is widely used to investigate β-cell biology, insulin secretion mechanisms, and diabetes pathophysiology. In rodent models, it helps dissect the distinct roles of Ins1 versus Ins2 in development and metabolic regulation. It also serves as an antigen in autoimmune diabetes studies to explore anti-insulin antibody responses. Additionally, recombinant Ins1 facilitates drug screening for insulin analogs and supports structural studies to engineer thermostable or long-acting insulin variants.
The availability of recombinant Ins1 has advanced diabetes research, particularly in understanding β-cell dysfunction and autoimmune targeting in type 1 diabetes. Its application extends to creating disease models and developing targeted therapies, underscoring its importance in both basic science and translational medicine.
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