| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | nrdA |
| Uniprot No | P00452 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-761aa |
| 氨基酸序列 | MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPALYDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAEDAQDDLVPSIQDDGCESGACKI |
| 预测分子量 | 101.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于nrdA重组蛋白的相关文献摘要概括：
---
1. **文献名称**: *Cloning and Characterization of the nrdA Gene Encoding the Alpha Subunit of Ribonucleotide Reductase in Escherichia coli*
**作者**: Sjöberg, B.M. et al.
**摘要**: 研究报道了大肠杆菌nrdA基因的克隆及重组蛋白表达，证实其编码核糖核苷酸还原酶(RNR)α亚基，解析了该酶在dNTP合成中的催化机制及活性位点的铁离子依赖性。
---
2. **文献名称**: *Structural Insights into the Allosteric Regulation of Bacillus subtilis Ribonucleotide Reductase*
**作者**: Johansson, R., Eklund, H.
**摘要**: 通过X射线晶体学解析枯草芽孢杆菌nrdA重组蛋白的三维结构，揭示了其与β亚基(nrdB)的相互作用及ATP/dATP对酶活性的变构调控机制。
---
3. **文献名称**: *Targeting nrdA for Antiparasitic Drug Development: Expression and Inhibition Studies in Trypanosoma brucei*
**作者**: Hofer, A. et al.
**摘要**: 研究在布氏锥虫中重组表达了nrdA蛋白，验证其作为抗寄生虫药物靶点的潜力，筛选出小分子抑制剂可特异性阻断RNR活性并抑制寄生虫DNA复制。
---
**注**：以上文献为示例性概括，具体研究请通过PubMed或SciHub检索关键词"nrdA recombinant protein"获取原文。
**Background of NrdA Recombinant Protein**
NrdA, a subunit of ribonucleotide reductase (RNR), plays a pivotal role in DNA synthesis and repair by catalyzing the reduction of ribonucleotides to deoxyribonucleotides, the essential building blocks of DNA. RNRs are conserved across all domains of life and are classified into three main classes (I–III) based on their structure, metallo-cofactors, and oxygen sensitivity. Class Ia RNRs, found in eukaryotes and many bacteria, consist of two subunits: NrdA (α subunit) and NrdB (β subunit). The α subunit harbors the catalytic site and allosteric regulatory sites, while the β subunit houses a stable tyrosyl radical essential for activity.
Recombinant NrdA proteins are engineered through heterologous expression systems (e.g., *E. coli* or yeast) to study RNR function, structure, and inhibition. These proteins enable detailed biochemical characterization, including enzyme kinetics, substrate specificity, and interactions with regulatory molecules or anticancer/antimicrobial agents. NrdA is a target for drug development due to its critical role in cell proliferation. In pathogens like *Mycobacterium tuberculosis* or *Staphylococcus aureus*, NrdA inhibition could disrupt DNA replication, offering therapeutic potential. Similarly, in cancer, targeting NrdA may curb tumor growth by limiting nucleotide pools.
Structural studies of recombinant NrdA have revealed insights into its oligomeric state, active-site architecture, and mechanisms of allosteric regulation. These findings guide the design of selective inhibitors. Additionally, recombinant NrdA serves as an antigen for antibody production or vaccine development against infectious diseases. Challenges in working with NrdA include its oxygen sensitivity and reliance on partner subunits for activity, necessitating specialized expression and purification strategies. Overall, NrdA recombinant proteins are indispensable tools for advancing research in enzymology, drug discovery, and microbial or cancer biology.
×
