| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETNK2 |
| Uniprot No | Q9NVF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAVPPSAPQPRASFHLRRHTPCPQCSW GMEEKAAASASCREPPGPPRAAAVAYFGISVDPDDILPGALRLIQELRPH WKPEQVRTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTELLVDRENE VRNFQLLRAHSCAPKLYCTFQNGLCYEYMQGVALEPEHIREPRLFRLIAL EMAKIHTIHANGSLPKPILWHKMHNYFTLVKNEINPSLSADVPKVEVLER ELAWLKEHLSQLESPVVFCHNDLLCKNIIYDSIKGHVRFIDYEYAGYNYQ AFDIGNHFNEFAGVNEVDYCLYPARETQLQWLHYYLQAQKGMAVTPREVQ RLYVQVNKFALASHFFWALWALIQNQYSTIDFDFLRYAVIRFNQYFKVKP QASALEMPK |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ETNK2重组蛋白的3篇参考文献示例(文献为虚构，仅供格式参考)：
1. **文献名称**：*Cloning and Functional Characterization of Recombinant ETNK2 in Cancer Metabolism*
**作者**：Smith A, et al.
**摘要**：研究报道了ETNK2重组蛋白的克隆与表达，揭示其通过调节磷脂代谢影响癌细胞增殖的分子机制。
2. **文献名称**：*Structural Insights into ETNK2 Catalytic Activity Using Recombinant Protein Crystallography*
**作者**：Chen L, et al.
**摘要**：通过重组ETNK2蛋白的晶体结构解析，阐明了其底物结合位点及催化乙醇胺磷酸化的关键氨基酸残基。
3. **文献名称**：*ETNK2 Recombinant Protein as a Therapeutic Target in Lipid Storage Disorders*
**作者**：Garcia R, et al.
**摘要**：验证了重组ETNK2蛋白在体外模型中纠正脂质代谢异常的能力，提示其作为治疗脂质蓄积疾病的潜在靶点。
(注：以上文献为示例，实际研究中需通过PubMed/Google Scholar等平台检索真实文献。)
ETNK2 (ethanolamine kinase 2) is a ubiquitously expressed enzyme that catalyzes the phosphorylation of ethanolamine to form phosphoethanolamine, a critical step in the Kennedy pathway for the biosynthesis of phosphatidylethanolamine (PE) and other phospholipids. These phospholipids are essential components of cellular membranes and play roles in signaling, membrane trafficking, and autophagy. ETNK2 shares structural homology with ethanolamine kinase 1 (ETNK1) but exhibits distinct tissue-specific expression patterns and regulatory mechanisms. While ETNK1 is primarily associated with liver metabolism, ETNK2 is implicated in broader cellular processes, including neuronal development and cancer progression. Dysregulation of ETNK2 has been linked to neurological disorders, such as developmental encephalopathy, and certain cancers, where altered phospholipid metabolism may drive tumor growth or metastasis.
Recombinant ETNK2 proteins are engineered to enable functional and structural studies. Typically produced in *E. coli* or mammalian expression systems, these proteins retain the kinase domain responsible for ethanolamine phosphorylation. Purification methods often involve affinity tags (e.g., His-tag) and chromatography to ensure high purity and enzymatic activity. Researchers use recombinant ETNK2 to investigate its kinetic properties, substrate specificity, and interactions with regulatory proteins or inhibitors. Structural analyses, including X-ray crystallography or cryo-EM, aim to elucidate its active site architecture and mechanisms of catalysis. Additionally, ETNK2 variants associated with disease mutations are studied to understand their impact on enzyme function and cellular lipid homeostasis. Such work may inform therapeutic strategies targeting phospholipid metabolism in cancer or neurological conditions.
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