| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | US12 |
| Uniprot No | P03170 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MSWALEMADTFLDTMRVGPRTYADVRDEINKRGREDREAARTAVHDPERPLLRSPGLLPEIAPNASLGVAHRRTGGTVTDSPRNPVTR |
| 预测分子量 | 11.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCMV US12重组蛋白的参考文献示例(内容基于学术文献的典型研究方向，仅供参考)：
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1. **文献名称**: *"Characterization of the Human Cytomegalovirus US12 Gene Family Reveals a Role in Virion Assembly"*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组表达HCMV US12蛋白，发现其与病毒粒子组装相关。利用哺乳动物细胞系统表达US12重组蛋白，结合免疫共沉淀技术，证实其与病毒衣壳蛋白的相互作用，提示US12在病毒形态发生中的潜在作用。
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2. **文献名称**: *"Recombinant US12 Protein of HCMV Modulates Innate Immune Response via TLR2 Signaling Pathway"*
**作者**: Lee H, et al.
**摘要**: 文章报道了在大肠杆菌中表达并纯化的HCMV US12重组蛋白，通过体外实验证明其可激活TLR2通路，诱导巨噬细胞分泌促炎因子，提示US12在宿主先天免疫调控中的机制。
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3. **文献名称**: *"Structural Insights into HCMV US12 Protein by Cryo-EM Analysis of Recombinant Complexes"*
**作者**: Zhang R, et al.
**摘要**: 通过冷冻电镜解析US12重组蛋白与宿主细胞受体的复合物结构，揭示了其独特的跨膜结构域特征，为设计靶向US12的抗病毒药物提供了结构基础。
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4. **文献名称**: *"Development of a US12-based ELISA for Serological Detection of HCMV Infection"*
**作者**: Müller S, et al.
**摘要**: 利用昆虫细胞系统表达HCMV US12重组蛋白，开发了高特异性的ELISA检测方法，验证了其在临床血清样本中诊断HCMV感染的灵敏度和准确性。
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**注**：以上文献为示例性质，实际文献需通过PubMed、Web of Science等数据库检索。建议使用关键词“HCMV US12 recombinant protein”或结合具体研究领域(如免疫调控、结构生物学)进一步筛选。
US12 is a viral regulatory protein encoded by the human cytomegalovirus (HCMV), a beta-herpesvirus with a large double-stranded DNA genome. The US12 gene family, unique to HCMV, comprises 10 transmembrane proteins (US12–US21) implicated in immune evasion and viral persistence. US12 specifically functions as an immunomodulatory protein that disrupts host antiviral responses. Studies suggest it downregulates major histocompatibility complex class I (MHC-I) molecules on infected cell surfaces, hindering antigen presentation to cytotoxic T-cells and enabling immune evasion. Additionally, US12 may interact with components of the endoplasmic reticulum, modulating protein trafficking or degradation pathways to favor viral replication.
Recombinant US12 protein is produced via molecular cloning and expression systems (e.g., E. coli, mammalian cells) for functional studies. Its purification often involves affinity tags (e.g., His-tag) to investigate structural features, binding partners, and mechanistic roles in immune suppression. Research on recombinant US12 has advanced understanding of HCMV pathogenesis, particularly its strategies to evade host immunity. This protein also serves as a potential target for antiviral therapies or vaccines aiming to restore immune recognition of infected cells. However, functional redundancy within the US12 gene family and incomplete characterization of its molecular interactions remain challenges. Current studies focus on delineating its precise mechanisms in MHC-I modulation and exploring its crosstalk with other viral proteins (e.g., US2. US11) to develop targeted interventions against HCMV-associated diseases in immunocompromised individuals.
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