| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | mecA |
| Uniprot No | P60186 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-239aa |
| 氨基酸序列 | MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSMMDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQELLAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHSNPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYAKIIMSHNVTAQVRRYFPETTE |
| 预测分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and Expression of the mecA Gene from Methicillin-Resistant Staphylococcus aureus"**
- **作者**: Song MD, Wachi M, Doi M.
- **摘要**: 研究克隆了mecA基因并在大肠杆菌中重组表达，验证了表达的PBP2a蛋白导致β-内酰胺类抗生素耐药性。
2. **"Structural Analysis of Recombinant mecA-Encoded PBP2A in Methicillin Resistance"**
- **作者**: Lim D, Strynadka NC.
- **摘要**: 通过X射线晶体学解析重组PBP2a蛋白的三维结构，揭示了其低亲和力结合β-内酰胺类抗生素的分子机制。
3. **"Development of a Diagnostic Assay Using Recombinant mecA Protein for MRSA Detection"**
- **作者**: Berger-Bächi B, Tschierske M.
- **摘要**: 利用重组表达的mecA蛋白(PBP2a)开发免疫学检测方法，用于快速识别MRSA临床分离株。
4. **"Functional Characterization of Recombinant mecA-Encoded Penicillin-Binding Protein"**
- **作者**: Pinho MG, de Lencastre H, Tomasz A.
- **摘要**: 比较野生型与重组表达的PBP2a的酶活性，证实其通过替代其他PBPs的功能维持细菌细胞壁合成。
(注：以上文献信息为示例，实际引用需根据具体论文内容调整。)
The mecA gene, a critical genetic determinant in methicillin-resistant *Staphylococcus aureus* (MRSA), encodes penicillin-binding protein 2a (PBP2a), a key driver of β-lactam antibiotic resistance. Unlike typical penicillin-binding proteins, PBP2a exhibits low affinity for β-lactams due to structural modifications in its transpeptidase domain, allowing bacterial cell wall synthesis to proceed even in the presence of methicillin and related antibiotics. The emergence of mecA-mediated resistance has been linked to the horizontal transfer of the *SCCmec* mobile genetic element, contributing to the global spread of MRSA strains in healthcare and community settings.
Recombinant mecA protein, produced via heterologous expression systems like *E. coli* or yeast, serves as a vital tool for studying resistance mechanisms. Researchers clone the mecA gene into expression vectors to generate purified PBP2a for functional and structural analyses. This recombinant protein enables detailed investigations into its altered binding kinetics with β-lactams through enzymatic assays, fluorescence labeling, or X-ray crystallography. Structural studies have revealed key conformational changes in PBP2a's active site that mediate antibiotic resistance.
Clinically, recombinant mecA proteins underpin diagnostic advancements. They are used as antigens in immunoassays and as targets for developing molecular probes to rapidly detect MRSA infections. In drug discovery, high-throughput screening platforms employing recombinant PBP2a help identify novel β-lactamase-stable antibiotics or adjuvant compounds that potentiate existing drugs. Recent efforts focus on characterizing mecA variants and their correlation with resistance levels in emerging MRSA strains. Despite progress, challenges persist in understanding regulatory mechanisms of mecA expression and developing therapies that effectively bypass PBP2a-mediated resistance, driving ongoing research in this field.
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