| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ldh1 |
| Uniprot No | P38139 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL |
| 预测分子量 | 43,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LDH1重组蛋白的模拟参考文献示例(内容为学术场景模拟，非真实文献)：
1. **《Expression and purification of recombinant human LDH1 for enzymatic activity analysis》**
- 作者：Zhang Y, et al.
- 摘要：研究利用大肠杆菌系统成功表达并纯化重组人LDH1蛋白，通过酶动力学实验验证其催化乳酸转化为丙酮酸的活性，为肿瘤代谢研究提供工具。
2. **《Optimization of LDH1 recombinant protein stability in vitro》**
- 作者：Wang L, et al.
- 摘要：探讨不同缓冲液条件对重组LDH1蛋白稳定性的影响，发现添加甘油和低温储存可显著延长其酶活性保存时间，适用于体外药物筛选实验。
3. **《Role of recombinant LDH1 in hepatocellular carcinoma cell metabolism》**
- 作者：Chen X, et al.
- 摘要：通过过表达重组LDH1.证明其增强肝癌细胞糖酵解能力并促进增殖，为靶向LDH1的癌症治疗策略提供理论依据。
4. **《Crystal structure analysis of prokaryotic-expressed LDH1 and inhibitor screening》**
- 作者：Kimura T, et al.
- 摘要：解析了原核表达的重组LDH1蛋白三维结构，并基于此筛选出小分子抑制剂，为开发新型代谢疾病药物奠定基础。
**说明**：以上为模拟生成的参考文献，实际文献建议通过PubMed、Web of Science或Google Scholar检索关键词“recombinant LDH1 protein”“lactate dehydrogenase expression”等获取。真实文献需注意出版年份及期刊权威性。
**Background of LDH1 Recombinant Protein**
Lactate dehydrogenase 1 (LDH1), a subunit of the lactate dehydrogenase (LDH) enzyme family, plays a critical role in cellular energy metabolism by catalyzing the interconversion of pyruvate and lactate. LDH exists as tetrameric isoforms composed of M (muscle) and H (heart) subunits, with LDH1 (H4) being predominantly expressed in heart muscle, red blood cells, and renal tissues. It serves as a key biomarker for tissue damage; elevated serum LDH1 levels are clinically associated with myocardial infarction, hemolytic anemia, and kidney disorders.
Recombinant LDH1 protein is produced using genetic engineering techniques, typically by expressing the *LDHA* or *LDHB* genes (encoding H subunits) in heterologous systems like *E. coli*, yeast, or mammalian cell cultures. This approach ensures high purity, specificity, and scalability, overcoming limitations of native protein extraction from tissues. Recombinant LDH1 retains enzymatic activity, enabling its use in functional studies, drug screening, and diagnostic assay development.
Research on LDH1 has expanded due to its emerging roles in cancer metabolism. Many tumors exhibit the "Warburg effect," relying on glycolysis even under aerobic conditions, with LDH isoforms facilitating lactate production. Inhibiting LDH1 has been explored as a therapeutic strategy to disrupt cancer cell proliferation. Additionally, recombinant LDH1 is employed in structural studies to elucidate its catalytic mechanism and allosteric regulation.
In summary, recombinant LDH1 provides a versatile tool for biomedical research, bridging fundamental biochemistry with clinical and therapeutic applications. Its production and characterization continue to advance our understanding of metabolic diseases, cancer biology, and biomarker-driven diagnostics.
×
