| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETFb |
| Uniprot No | P38117 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAELRVLVAVKRVIDYAVKIRVKPDRTGVV TDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALA MGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDD CNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAV VTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDP PQRTAGVKVETTEDLVAKLKEIGRI |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ETF-β(Electron Transfer Flavoprotein Beta Subunit)重组蛋白的3篇参考文献示例，内容基于典型研究方向整理：
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1. **文献名称**：*Production and Characterization of Recombinant Human Electron Transfer Flavoprotein Beta Subunit*
**作者**：Smith J, et al.
**摘要**：本研究描述了通过大肠杆菌表达系统高效制备重组人ETF-β亚基的方法，并验证其与天然蛋白相似的二级结构及电子传递活性，为代谢疾病研究提供工具。
2. **文献名称**：*Functional Analysis of ETF-β Mutations in Glutaric Acidemia Type II*
**作者**：Chen L, et al.
**摘要**：通过重组ETF-β蛋白及突变体实验，揭示了特定氨基酸位点突变导致电子传递功能障碍的机制，解释了Ⅱ型戊二酸血症的分子病理。
3. **文献名称**：*Crystallographic Study of ETF-β in Mitochondrial Fatty Acid Oxidation*
**作者**：Wang Y, et al.
**摘要**：利用重组ETF-β蛋白进行X射线晶体学研究，解析其与脱氢酶相互作用的分子结构，阐明其在脂肪酸氧化中的关键作用。
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**说明**：
- ETF-β是线粒体电子传递黄素蛋白的亚基，常与代谢疾病(如戊二酸血症)相关，重组蛋白研究多聚焦于结构、功能及疾病模型应用。
- 实际文献需通过PubMed/Google Scholar以“ETF-beta recombinant”、“ETFB expression”等关键词检索获取，建议结合具体研究领域筛选。
**Background of ETFβ Recombinant Protein**
ETFβ (Electron Transfer Flavoprotein β-subunit) recombinant protein is a key component of the electron transfer flavoprotein complex, which plays a critical role in mitochondrial energy metabolism. The ETF complex, composed of α (ETFα) and β (ETFβ) subunits, acts as an electron carrier in the mitochondrial matrix, shuttling electrons from fatty acid β-oxidation and amino acid catabolism to the ubiquinone pool via ETF-ubiquinone oxidoreductase (ETF-QO). This process is essential for ATP production, particularly during fasting or prolonged exercise when fatty acids are a major energy source.
Mutations in the *ETFB* gene, encoding ETFβ, are linked to multiple acyl-CoA dehydrogenase deficiency (MADD), a rare autosomal recessive disorder characterized by metabolic crises, muscle weakness, and organ dysfunction. Recombinant ETFβ protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian cell systems) to study its structure-function relationships, develop diagnostic tools, or explore therapeutic strategies for MADD. Its recombinant form allows for high purity and scalability, enabling research into enzyme replacement therapies or gene therapy vectors.
Additionally, ETFβ recombinant protein serves as a valuable tool in biochemical assays to investigate electron transfer mechanisms, screen potential drug candidates, or model mitochondrial dysfunction in metabolic diseases. Studies leveraging recombinant ETFβ have advanced understanding of flavoprotein interactions and their roles in redox homeostasis, offering insights into broader cellular energy regulation pathways.
Overall, ETFβ recombinant protein bridges basic research and clinical applications, providing a foundation to address metabolic disorders and refine targeted interventions for mitochondrial electron transport chain deficiencies.
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