| 纯度 | >90%SDS-PAGE. |
| 种属 | Saccharomyces |
| 靶点 | PNC1 |
| Uniprot No | P53184 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MKTLIVVDMQNDFISPLGSLTVPKGEELINPISDLMQDADRDWHRIVVTRDWHPSRHISFAKNHKDKEPYSTYTYHSPRPGDDSTQEGILWPVHCVKNTWGSQLVDQIMDQVVTKHIKIVDKGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIVGVALEYCVKATAISAAELGYKTTVLLDYTRPISDDPEVINKVKEELKAHNINVVDK |
| 预测分子量 | 25.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PNC1重组蛋白的3篇模拟参考文献(内容基于公开领域知识综合整理，非真实文献)：
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1. **文献名称**: *"PNC1-mediated NAD+ biosynthesis regulates stress resistance and longevity in Saccharomyces cerevisiae"*
**作者**: Anderson, R.M., et al.
**摘要**: 研究通过重组PNC1蛋白在酵母中的过表达，证明其烟酰胺酶活性可促进NAD+合成，增强氧化应激抗性并延长酵母寿命，机制依赖于Sir2去乙酰化酶的激活。
2. **文献名称**: *"Characterization of recombinant human PNC1 protein: enzymatic properties and role in cellular senescence"*
**作者**: Benedict, C., et al.
**摘要**: 通过体外表达纯化人源PNC1重组蛋白，分析其酶动力学参数及最适pH条件，并发现其通过调节NAD+水平延缓哺乳动物细胞衰老表型。
3. **文献名称**: *"Functional analysis of PNC1 orthologs in Caenorhabditis elegans: implications for aging and metabolic regulation"*
**作者**: Smith, J.L., & Johnson, T.E.
**摘要**: 利用重组线虫PNC1蛋白进行功能研究，发现其通过降解烟酰胺调控胰岛素信号通路，影响寿命和热量限制效应。
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注：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of PNC1 Recombinant Protein**
The PNC1 (nicotinamidase 1) gene encodes a critical enzyme involved in the nicotinamide salvage pathway, which regulates cellular levels of nicotinamide (NAM) and influences NAD+ metabolism. Initially identified in yeast, PNC1 catalyzes the deamidation of NAM to nicotinic acid (NA), a precursor for NAD+ biosynthesis. NAD+ is a central cofactor in redox reactions and a key substrate for sirtuins, a family of proteins linked to aging, stress resistance, and metabolic regulation.
PNC1 gained prominence due to its role in longevity. Studies in model organisms, such as *Saccharomyces cerevisiae* and *Caenorhabditis elegans*, demonstrated that PNC1 overexpression extends lifespan, particularly under stress conditions like calorie restriction. This effect is attributed to its ability to lower intracellular NAM levels, thereby reducing inhibition of sirtuins (e.g., Sir2) and enhancing their deacetylase activity. Sirtuin activation promotes genomic stability, stress response, and metabolic efficiency, mechanisms conserved across species.
Recombinant PNC1 protein is produced using heterologous expression systems (e.g., *E. coli* or yeast) to study its enzymatic properties, structure-function relationships, and therapeutic potential. Its recombinant form enables high-purity, scalable production for biochemical assays, structural studies (e.g., crystallography), and preclinical research.
Interest in PNC1 extends to human health, as NAD+ depletion is linked to aging-related diseases (e.g., neurodegeneration, metabolic disorders). While humans lack a direct PNC1 homolog, insights from its recombinant protein inform strategies to modulate NAD+ pathways, such as developing small-molecule activators or NAM-cleaving enzymes. Current research explores PNC1-inspired interventions to counteract age-associated decline, emphasizing its translational relevance in biogerontology and precision medicine.
In summary, PNC1 recombinant protein serves as a vital tool to dissect NAD+ biology and aging mechanisms, bridging fundamental discoveries to potential therapies for age-related diseases.
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