| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | VP3 |
| Uniprot No | P54095 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-120aa |
| 氨基酸序列 | MNALQEDTPPGPSTVFRPPTSSRPLETPHCEIRIGIAGITITLSLCGCAN ARAPTLRSATADSSESTGFKNAPDLRTDQPKPPSKKRSCDPSEYRVSELK ENLITTTPSRPRTARRCIRL |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VP3重组蛋白的3篇参考文献示例(注：部分文献为虚构示例，实际引用时请核实真实来源)：
1. **《Expression and Immunogenicity of Recombinant VP3 Protein of Infectious Bursal Disease Virus》**
- 作者：Li, X. et al.
- 摘要：研究通过大肠杆菌表达系统成功获得IBDV的VP3重组蛋白，动物实验表明其能诱导鸡体产生特异性抗体，为亚单位疫苗开发提供依据。
2. **《Structural Characterization of VP3 in Avian Reovirus Capsid Assembly》**
- 作者：Wang, Y. & Zhang, H.
- 摘要：利用冷冻电镜解析禽呼肠孤病毒VP3蛋白的三维结构，揭示其与VP1协同作用促进病毒衣壳组装的关键机制。
3. **《VP3-Based ELISA for Serodiagnosis of Foot-and-Mouth Disease Virus Infection》**
- 作者：Patel, R. et al.
- 摘要：开发基于重组VP3蛋白的ELISA检测方法，证实其对口蹄疫病毒抗体的高特异性和敏感性，适用于大规模血清学筛查。
4. **《Baculovirus-mediated VP3 Expression and Its Antiviral Activity in Shrimp》**
- 作者：Chen, L. et al.
- 摘要：在昆虫细胞中表达对虾白斑综合征病毒VP3蛋白，证实其可激活宿主免疫通路，降低病毒感染率，具有潜在治疗应用价值。
(注：如需真实文献，建议通过PubMed或Web of Science检索关键词“VP3 recombinant protein”或结合具体病毒名称筛选。)
**Background of VP3 Recombinant Protein**
The VP3 recombinant protein is derived from the VP3 structural protein of the **infectious bursal disease virus (IBDV)**, a double-stranded RNA virus belonging to the *Avibirnavirus* genus. IBDV primarily infects young chickens, targeting the bursa of Fabricius, a critical immune organ, leading to immunosuppression and high mortality in severe cases. VP3 is a key component of the viral capsid, playing essential roles in virus assembly, stability, and interactions with host cells.
Recombinant VP3 is produced using **genetic engineering techniques**, where the VP3 gene is cloned into expression vectors (e.g., bacterial, insect, or mammalian systems) to enable large-scale protein synthesis. This approach ensures high purity and avoids risks associated with handling live viruses.
VP3 has garnered attention in research and diagnostics due to its **immunogenic properties**. It participates in forming virus-like particles (VLPs) and serves as a target for antibody detection in serological assays. Studies also explore its role in viral replication and host immune evasion, particularly its interaction with VP1 (viral RNA polymerase) and involvement in viral RNA packaging.
Additionally, VP3 recombinant protein is investigated for **vaccine development**, either as a subunit vaccine component or in combination with other viral proteins to enhance protective immunity. Its conserved structure across IBDV strains makes it a candidate for broad-spectrum diagnostic tools and vaccines.
In summary, VP3 recombinant protein serves as a vital tool for understanding IBDV pathogenesis, improving diagnostics, and advancing vaccine strategies, contributing to poultry health and disease control globally.
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