| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | rtcB |
| Uniprot No | P46850 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG |
| 预测分子量 | 47.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rtcB重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*RtcB is the RNA ligase component of an Escherichia coli RNA repair operon*
**作者**：Chakravarty, A. K., & Shuman, S.
**摘要**：该研究首次报道了大肠杆菌中rtcB基因编码的RtcB蛋白是一种ATP依赖的RNA连接酶，参与RNA修复。通过重组蛋白实验，证实RtcB可将2',3'-环磷酸末端与5'-羟基末端的RNA链连接，揭示了其在细菌RNA损伤修复中的关键作用。
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2. **文献名称**：*Crystal structure of RtcB in complex with RNA and GTP reveals the molecular mechanism of RNA ligation*
**作者**：Maughan, W. P., & Shuman, S.
**摘要**：本研究解析了重组RtcB蛋白与RNA及GTP的复合物晶体结构，揭示了其依赖金属离子的催化机制。结构分析表明，RtcB通过GTP水解激活连接反应，为理解其RNA修复的分子机制提供了结构基础。
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3. **文献名称**：*Human RtcB is a tRNA ligase required for unfolded protein response signaling*
**作者**：Jurkin, J., et al.
**摘要**：文章发现人源重组RtcB蛋白在内质网应激反应中通过连接剪切后的tRNA分子，维持未折叠蛋白反应(UPR)信号通路的活性。功能实验表明，RtcB缺失导致细胞对ER应激敏感性增加，提示其在哺乳动物细胞应激适应中的关键作用。
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这些研究涵盖了RtcB重组蛋白的酶学功能、结构机制及生理意义，为RNA修复和细胞应激领域提供了重要依据。
RtcB is a highly conserved RNA ligase that plays a critical role in RNA repair and stress response pathways across bacteria, archaea, and eukaryotes. Initially identified in *Escherichia coli*, RtcB homologs have been found in diverse organisms, including humans (hsRtcB). This enzyme catalyzes the ligation of RNA strands with 2′,3′-cyclic phosphate and 5′-OH termini, a reaction essential for repairing damaged RNA or processing specialized RNA molecules. Its activity is metal ion-dependent, requiring Mn²⁺ or Mg²⁺, and involves a unique catalytic mechanism that bypasses the need for ATP, instead utilizing a covalent RtcB-guanylate intermediate.
In eukaryotes, RtcB participates in multiple RNA quality control pathways. It is involved in the unconventional splicing of XBP1 mRNA during the unfolded protein response (UPR), tRNA maturation, and the resolution of RNA breaks caused by cellular stress. Studies have linked RtcB dysfunction to neurological disorders and cancer, highlighting its importance in maintaining RNA integrity under stress conditions.
Recombinant RtcB proteins are engineered for research and therapeutic applications, typically expressed in *E. coli* or mammalian systems. Structural studies reveal a conserved core domain with a unique fold housing the active site, while flexible regions mediate substrate recognition. Purified recombinant RtcB retains enzymatic activity in vitro, enabling studies on RNA repair mechanisms, stress granule dynamics, and tRNA modification processes. Its role in RNA surveillance and stress adaptation makes it a potential target for therapies addressing neurodegenerative diseases or conditions involving chronic endoplasmic reticulum stress. Ongoing research aims to unravel its regulatory networks and exploit its ligase activity for biotechnology tools, such as RNA nanotechnology or synthetic biology applications.
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