| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | plcA |
| Uniprot No | Q8NI17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-732aa |
| 氨基酸序列 | MMWTWALWMLPSLCKFSLAALPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIAKTEPPKIFRVKPVLGIKRMIQIEWIKPELAPVSSDLKYTLRFRTVNSTSWMEVNFAKNRKDKNQTYNLTGLQPFTEYVIALRCAVKESKFWSDWSQEKMGMTEEEAPCGLELWRVLKPAEADGRRPVRLLWKKARGAPVLEKTLGYNIWYYPESNTNLTETMNTTNQQLELHLGGESFWVSMISYNSLGKSPVATLRIPAIQEKSFQCIEVMQACVAEDQLVVKWQSSALDVNTWMIEWFPDVDSEPTTLSWESVSQATNWTIQQDKLKPFWCYNISVYPMLHDKVGEPYSIQAYAKEGVPSEGPETKVENIGVKTVTITWKEIPKSERKGIICNYTIFYQAEGGKGFSKTVNSSILQYGLESLKRKTSYIVQVMASTSAGGTNGTSINFKTLSFSVFEIILITSLIGGGLLILIILTVAYGLKKPNKLTHLCWPTVPNPAESSIATWHGDDFKDKLNLKESDDSVNTEDRILKPCSTPSDKLVIDKLVVNFGNVLQEIFTDEARTGQENNLGGEKNGYVTCPFRPDCPLGKSFEELPVSPEIPPRKSQYLRSRMPEGTRPEAKEQLLFSGQSLVPDHLCEEGAPNPYLKNSVTAREFLVSEKLPEHTKGEV |
| 预测分子量 | 82,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与plcA重组蛋白相关的文献示例(内容为模拟概括，实际文献需通过学术数据库验证)：
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1. **文献名称**：*"Cloning and expression of the plcA gene encoding Listeria monocytogenes phosphatidylinositol-specific phospholipase C"*
**作者**：Schluter D. et al.
**摘要**：报道了单核细胞增生李斯特菌plcA基因的克隆及在大肠杆菌中的重组表达，验证了重组蛋白的磷脂酶C活性及其在细菌致病性中的作用。
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2. **文献名称**：*"Functional characterization of recombinant PlcA in Listeria virulence"*
**作者**：Vázquez-Boland J.A. et al.
**摘要**：通过体外实验证明plcA重组蛋白可特异性水解宿主细胞膜磷脂，并与其他毒力因子协同促进李斯特菌的细胞内寄生和扩散。
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3. **文献名称**：*"Immunogenicity of recombinant PlcA as a vaccine candidate against listeriosis"*
**作者**：Notting C.M. et al.
**摘要**：评估了plcA重组蛋白在小鼠模型中的免疫保护效果，发现其可诱导Th1型免疫反应并降低李斯特菌感染后的组织载菌量。
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**备注**：以上为基于plcA研究的典型方向模拟，实际文献请通过PubMed/Web of Science等平台以关键词“plcA recombinant”或“Listeria phospholipase C”检索最新或高引论文。
**Background of PLC-A Recombinant Protein**
Phospholipase C-A (PLC-A) is a key enzyme involved in cellular signaling pathways, particularly in the hydrolysis of membrane phospholipids such as phosphatidylinositol 4.5-bisphosphate (PIP2) to generate secondary messengers like inositol trisphosphate (IP3) and diacylglycerol (DAG). These molecules regulate critical processes, including calcium release, protein kinase C activation, and cell proliferation. PLC-A, a member of the PLC family, is often studied in the context of bacterial pathogenesis, as homologs like *Listeria monocytogenes* PlcA contribute to host cell invasion and immune evasion by disrupting membrane integrity.
Recombinant PLC-A protein is engineered through genetic cloning, where the *plcA* gene is inserted into expression vectors (e.g., *E. coli* or yeast systems) to enable large-scale production. This approach allows for high purity and consistency, bypassing risks associated with native pathogen-derived extraction. The recombinant form retains enzymatic activity, making it valuable for studying host-pathogen interactions, signal transduction mechanisms, and potential therapeutic targets.
Applications include vaccine development, as PLC-A’s role in virulence highlights its potential as an antigen. It is also used in diagnostic assays to detect pathogen-specific antibodies. Additionally, structural studies of recombinant PLC-A provide insights into substrate binding and catalytic mechanisms, aiding inhibitor design. Its production under controlled conditions ensures safety for laboratory use while enabling modifications (e.g., tagging for purification or tracking). Overall, PLC-A recombinant protein serves as a versatile tool in microbiology, immunology, and cell biology research.
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