| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AFAP1 |
| Uniprot No | Q8N556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-730aa |
| 氨基酸序列 | MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFDVKDHA QKQETANSLP APPQMPLPEI PQPWLPPDSG PPPLPTSSLP EGYYEEAVPL SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKTRHQ WPSEEASMDL VKDAKICAFL LRKKRFGQWT KLLCVIKDTK LLCYKSSKDQ QPQMELPLQG CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW LKVIKEAYSG CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT CGYLNVLSNS RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG LDSKHPLTFR LLRNGQEVAV LEASSSEDMG RWIGILLAET GSSTDPEALH YDYIDVEMSA SVIQTAKQTF CFMNRRVISA NPYLGGTSNG YAHPSGTALH YDDVPCINGS LKGKKPPVAS NGVTGKGKTL SSQPKKADPA AVVKRTGSNA AQYKYGKNRV EADAKRLQTK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE VNAGRKPQAI LEEKLKQLEE ECRQKEAERV SLELELTEVK ESLKKALAGG VTLGLAIEPK SGTSSPQSPV FRHRTLENSP ISSCDTSDTE GPVPVNSAAV LKKSQAAPGS SPCRGHVLRK AKEWELKNGT |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AFAP1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"AFAP1 is a scaffold protein required for actin stress fiber formation and cancer cell migration"*
**作者**: Smith ER, et al.
**摘要**: 本研究利用重组AFAP1蛋白验证其作为支架蛋白的功能，发现其通过结合Src激酶和肌动蛋白纤维调控细胞骨架重组，促进肿瘤细胞迁移。重组蛋白实验表明AFAP1缺失会抑制应力纤维形成。
2. **文献名称**: *"Characterization of AFAP1-AS1 lncRNA interaction with AFAP1 protein in pancreatic cancer progression"*
**作者**: Zhao L, et al.
**摘要**: 通过重组AFAP1蛋白的体外结合实验，揭示了AFAP1-AS1长链非编码RNA与AFAP1的直接互作机制，证明该互作激活Rho信号通路，驱动胰腺癌细胞侵袭转移。
3. **文献名称**: *"Recombinant AFAP1 expression in HEK293 cells: Purification and functional analysis in cytoskeletal remodeling"*
**作者**: Kim H, Park JH
**摘要**: 报道了AFAP1重组蛋白在HEK293细胞中的高效表达与纯化方案，并证明其通过调控Arp2/3复合体增强肌动蛋白分支，为研究AFAP1在细胞运动中的作用提供工具。
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注：上述文献为示例性质，实际文献需通过学术数据库检索确认。如需具体文章，建议在PubMed或Web of Science中以“AFAP1 recombinant”为关键词进一步筛选。
AFAP1 (Actin Filament-Associated Protein 1) is a cytoskeletal adaptor protein that plays a role in regulating actin dynamics and cellular signaling pathways. It contains multiple functional domains, including SH3-binding motifs and phosphorylation sites, enabling interactions with signaling molecules such as SRC kinase. AFAP1 is implicated in modulating cell motility, adhesion, and cytoskeletal reorganization by bridging actin filaments to signaling complexes. Its involvement in SRC-mediated pathways links it to processes like cell proliferation, invasion, and tumor progression, particularly in cancers (e.g., pancreatic, breast).
Recombinant AFAP1 protein is engineered for in vitro studies to dissect its molecular functions and interactions. Produced via bacterial or eukaryotic expression systems, it retains key domains for binding assays, structural studies, or inhibitor screening. Researchers use it to explore AFAP1’s role in diseases, including cancer metastasis and chronic inflammatory conditions, where aberrant cytoskeletal regulation contributes to pathology. Additionally, recombinant AFAP1 aids in developing diagnostic tools or therapeutic strategies targeting AFAP1-associated pathways. Its purity and activity are validated via SDS-PAGE, Western blot, or functional assays, ensuring reliability for mechanistic or translational research. Studies utilizing recombinant AFAP1 continue to clarify its dual roles as a scaffold protein and potential biomarker in disease contexts.
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