| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | tktA |
| Uniprot No | P27302 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663aa |
| 氨基酸序列 | MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL |
| 预测分子量 | 88.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及tktA重组蛋白的文献摘要概括：
1. **《Enhanced production of shikimic acid by overexpression of tktA in Escherichia coli》**
- 作者：Li, Y., et al.
- 摘要：研究通过在大肠杆菌中重组表达tktA(转酮醇酶)，优化磷酸戊糖途径，显著提高了莽草酸产量，为芳香族化合物生物合成提供策略。
2. **《Purification and characterization of recombinant transketolase (tktA) from Bacillus subtilis》**
- 作者：Zhang, H., et al.
- 摘要：报道了枯草芽孢杆菌tktA基因的克隆、重组表达及蛋白纯化，分析了该酶的动力学特性及在辅酶硫胺素焦磷酸依赖催化中的作用。
3. **《Metabolic engineering of Saccharomyces cerevisiae using tktA for improved xylose utilization》**
- 作者：Wang, Q., et al.
- 摘要：通过引入重组tktA增强酵母磷酸戊糖途径通量，提升木糖代谢效率，为生物燃料生产中的底物利用优化提供依据。
注：以上文献为示例，实际文献需通过数据库(如PubMed、ScienceDirect)检索确认具体信息。
**Background of TktA Recombinant Protein**
TktA, encoded by the *tktA* gene, is a transketolase enzyme involved in the pentose phosphate pathway (PPP), a critical metabolic route in prokaryotic and eukaryotic organisms. This enzyme catalyzes the reversible transfer of a two-carbon ketol group between sugar phosphates, playing a key role in nucleotide biosynthesis, NADPH production, and carbon metabolism. In bacteria such as *Escherichia coli*, TktA is essential for maintaining redox balance and generating ribose-5-phosphate, a precursor for DNA/RNA synthesis.
Recombinant TktA protein is produced by cloning the *tktA* gene into expression vectors (e.g., plasmid systems) and overexpressing it in heterologous hosts like *E. coli*. This approach enables large-scale production of the enzyme with high purity, often facilitated by affinity tags (e.g., His-tag) for simplified purification. Recombinant TktA retains its native enzymatic activity, making it valuable for biochemical studies, such as substrate specificity analyses, kinetic assays, and structural characterization (e.g., X-ray crystallography).
Research on TktA has implications in metabolic engineering and biotechnology. For instance, enhancing PPP flux via TktA overexpression can improve microbial production of biofuels, amino acids, or pharmaceuticals. Conversely, TktA inhibition is explored as a strategy to disrupt bacterial metabolism, potentially aiding antibiotic development. Additionally, TktA’s role in oxidative stress response links it to studies on aging, cancer, and metabolic disorders.
Overall, TktA recombinant protein serves as a versatile tool for understanding fundamental biochemistry, optimizing industrial processes, and developing therapeutic interventions. Its study bridges gaps between microbial physiology, enzymology, and applied biotechnology.
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