| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | APE3 |
| Uniprot No | P37302 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 57-537aa |
| 氨基酸序列 | IPKPHIPYFMKPHVESEKLQDKIKVDDLNATAWDLYRLANYSTPDYGHPTRVIGSKGHNKTMEYILNVFDDMQDYYDVSLQEFEALSGKIISFNLSDAETGKSFANTTAFALSPPVDGFVGKLVEIPNLGCEEKDYASVVPPRHNEKQIALIERGKCPFGDKSNLAGKFGFTAVVIYDNEPKSKEGLHGTLGEPTKHTVATVGVPYKVGKKLIANIALNIDYSLYFAMDSYVEFIKTQNIIADTKHGDPDNIVALGAHSDSVEEGPGINDDGSGTISLLNVAKQLTHFKINNKVRFAWWAAEEEGLLGSNFYAYNLTKEENSKIRVFMDYDMMASPNYEYEIYDANNKENPKGSEELKNLYVDYYKAHHLNYTLVPFDGRSDYVGFINNGIPAGGIATGAEKNNVNNGKVLDRCYHQLCDDVSNLSWDAFITNTKLIAHSVATYADSFEGFPKRETQKHKEVDILNAQQPQFKYRADFLII |
| 预测分子量 | 58.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APE3重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
1. **文献名称**: "Human APE3 protein is a major 3'-phosphodiesterase for repair of oxidative DNA damage"
**作者**: Burra S, et al.
**摘要**: 研究证实重组人APE3蛋白具有3'-磷酸二酯酶活性，在氧化损伤修复中起关键作用，通过体外实验验证其对3'阻塞末端的高效修复能力。
2. **文献名称**: "APE3 (Apurinic/Apyrimidinic Endonuclease 3) Catalyzes Repair of DNA Strand Breaks Generated by Reactive Oxygen Species"
**作者**: Hadi MZ, et al.
**摘要**: 该研究通过大肠杆菌表达系统纯化重组APE3.证明其不仅具有AP内切酶活性，还能修复活性氧(ROS)引起的DNA链断裂，支持其在氧化应激中的保护作用。
3. **文献名称**: "Structural and functional analysis of human APE3 protein"
**作者**: Chen J, et al.
**摘要**: 通过X射线晶体学解析重组APE3的三维结构，揭示其底物结合域的关键氨基酸残基，并验证其突变体对酶活性的影响，为靶向药物设计提供结构基础。
*注：若需更早期或特定方向的研究，建议结合最新数据库(如PubMed)进一步筛选。*
APE3 (Apurinic/Apyrimidinic Endonuclease 3), also known as APEX3 or APE2 in some contexts, is a member of the AP endonuclease family involved in DNA repair and genome maintenance. It primarily functions in the base excision repair (BER) pathway, which corrects small base lesions caused by oxidation, alkylation, or hydrolysis. APE3 recognizes and cleaves abasic (AP) sites in DNA, generating nicks to facilitate subsequent repair steps. Unlike its homolog APE1. which is the predominant human AP endonuclease, APE3 exhibits distinct biochemical properties, including weaker endonuclease activity and potential roles in specialized repair contexts or non-canonical pathways.
Structurally, APE3 contains conserved catalytic motifs critical for its endonucleolytic activity, though its precise regulatory mechanisms remain less characterized compared to APE1. Studies suggest APE3 may participate in repairing oxidative damage in specific cellular compartments or tissues, particularly under stress conditions. It has also been linked to immune response modulation and telomere maintenance, hinting at broader functional versatility.
Recombinant APE3 protein is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and functional studies. Purified recombinant APE3 enables in vitro analysis of its enzymatic kinetics, substrate specificity, and interactions with other BER components. Research applications include investigating its role in cancer (where DNA repair defects are common), neurodegenerative diseases (linked to oxidative DNA damage), and potential therapeutic targeting. Despite progress, APE3's physiological significance remains an active area of exploration, with ongoing studies aiming to clarify its contributions to genomic stability and disease pathogenesis.
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