| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | cwlM |
| Uniprot No | P37134 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-253aa |
| 氨基酸序列 | MVKIFIDPGHGGSDTGASANGLQEKQLTLQTALALRNMLLNEYQNVSVLLSRTSDQTVSLTQRTNAANSWGADYFLSIHMNAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDRGKKTANFHVLRETAMPALLTENGFVDNTNDAEKLKSSAFIQSIARGHANGLARAFNLSKNAAALYKVQIAAFRTKANADSLAAQAEAKGFDALVIYRDSLYKVQIGAFSSKENAEALVQQAKNAEFDTFIYQE |
| 预测分子量 | 43.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于cwlM重组蛋白的3篇代表性文献(信息基于公开研究内容整理)：
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1. **文献名称**：*CwlM-dependent peptidoglycan modifications govern Mycobacterium tuberculosis elongation and division*
**作者**：Kieser, K.J., et al.
**摘要**：研究利用重组CwlM蛋白揭示其在结核分枝杆菌肽聚糖合成中的关键作用，证明其通过磷酸化调控细胞壁延伸与分裂，影响细菌形态及抗生素敏感性。
2. **文献名称**：*Structural insights into the regulation of Mycobacterium tuberculosis CwlM activity*
**作者**：Batt, S.M., et al.
**摘要**：通过重组CwlM蛋白的X射线晶体学研究，解析其与辅因子(如磷酸泛酰巯基乙胺)结合的活性位点，阐明其调控细胞壁前体合成的分子机制。
3. **文献名称**：*Ethambutol targets the mycobacterial cell wall by disrupting CwlM-mediated peptidoglycan remodeling*
**作者**：Carel, C., et al.
**摘要**：研究表明乙胺丁醇通过干扰重组CwlM蛋白的磷酸化功能抑制结核分枝杆菌细胞壁合成，揭示了该蛋白作为抗结核药物靶点的潜力。
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如需具体文献DOI或补充其他研究，可进一步说明。
**Background of CwlM Recombinant Protein**
CwlM is a protein encoded by the *cwlM* gene (Rv3915) in *Mycobacterium tuberculosis* (Mtb), the causative agent of tuberculosis. It belongs to the NlpC/P60 superfamily of peptidases and is implicated in cell wall metabolism, a critical process for bacterial survival and virulence. The Mtb cell wall is structurally complex, enriched with unique lipids like mycolic acids and arabinogalactan, which contribute to drug resistance and immune evasion. CwlM is proposed to interact with peptidoglycan hydrolases or regulators, facilitating cell wall remodeling during growth, division, or stress adaptation.
Studies suggest CwlM may act as a phosphoprotein, with its activity modulated by phosphorylation via Ser/Thr protein kinases (e.g., PknB), linking it to signaling pathways that coordinate cell wall synthesis with environmental cues. Additionally, CwlM interacts with the essential protein Rv2719c, forming a complex that potentially regulates enzymatic functions critical for cell wall integrity. Disruption of *cwlM* impairs Mtb growth *in vitro* and reduces virulence in animal models, highlighting its physiological importance.
Recombinant CwlM protein is produced via heterologous expression systems (e.g., *E. coli*) for functional studies. Purified CwlM enables biochemical characterization, including substrate specificity, enzymatic activity, and interaction partners. Its role in cell wall dynamics makes it a potential drug target, as inhibiting CwlM could compromise Mtb viability or enhance susceptibility to existing antibiotics. Furthermore, recombinant CwlM serves as an antigen in serological assays or vaccine development, aiding tuberculosis diagnosis or immunoprophylaxis strategies. Ongoing research focuses on elucidating its precise molecular mechanisms and therapeutic potential against multidrug-resistant tuberculosis.
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