| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | entC2 |
| Uniprot No | P34071 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-266aa |
| 氨基酸序列 | ESQPDPTPDELHKSSEFTGTMGNMKYLYDDHYVSATKVMSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLIRVYENKRNTISFEVQTDKKSVTAQELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEVHLTTKNG |
| 预测分子量 | 27.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **entC2重组蛋白** 的3篇示例参考文献(注：文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**: *Cloning and Functional Characterization of entC2 in Escherichia coli*
**作者**: Smith A, Johnson B
**摘要**: 本研究通过PCR扩增大肠杆菌中的entC2基因，构建重组表达载体并成功在E. coli BL21中表达。重组蛋白EntC2经纯化后验证其参与铁载体肠菌素(enterobactin)的生物合成，酶活实验表明其催化分支酸转化为2.3-二羟基苯甲酸的关键步骤。
2. **文献名称**: *Structural Insights into EntC2 as a Siderophore Synthase*
**作者**: Lee C, et al.
**摘要**: 通过X射线晶体学解析了重组EntC2蛋白的三维结构，揭示了其活性位点的关键氨基酸残基。研究结合突变实验证明，EntC2通过特定的底物结合口袋催化铁载体的合成，为开发抗菌药物靶点提供理论依据。
3. **文献名称**: *Heterologous Expression of entC2 in Yeast for Siderophore Production*
**作者**: Wang D, et al.
**摘要**: 将entC2基因导入毕赤酵母系统进行异源表达，优化发酵条件后获得高产量重组EntC2蛋白。该蛋白在体外成功合成铁载体，并应用于重金属吸附实验，展示了其在环境修复中的潜在应用价值。
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如需真实文献，建议通过 **PubMed/Google Scholar** 检索关键词：*entC2 recombinant protein, enterobactin biosynthesis, siderophore enzymes*。
**Background of entC2 Recombinant Protein**
The entC2 gene is a component of bacterial secondary metabolite biosynthetic pathways, particularly associated with iron acquisition systems in certain Gram-negative bacteria. It is implicated in the synthesis of enterobactin, a high-affinity siderophore critical for scavenging iron under iron-limited conditions. Enterobactin, a catechol-type siderophore, is produced via the *ent* gene cluster (*entA–entF*), where entC2 (or related homologs) encodes enzymes involved in the assembly of the siderophore backbone. These siderophores enable bacteria to compete for iron in host environments, playing a vital role in bacterial virulence and survival during infection.
Recombinant entC2 protein is engineered through molecular cloning, where the entC2 gene is inserted into expression vectors (e.g., *E. coli* plasmids) under inducible promoters (e.g., T7 or lacZ). Host cells like *E. coli* BL21(DE3) are commonly used for overexpression, leveraging their high protein yield and ease of cultivation. Post-expression, the recombinant protein is purified via affinity chromatography (e.g., His-tag/Ni-NTA systems) and validated through SDS-PAGE or Western blot.
Studies on entC2 recombinant protein focus on elucidating its enzymatic role in siderophore biosynthesis, substrate specificity, and interaction with other pathway components. This protein is also a target for antimicrobial strategies aiming to disrupt bacterial iron uptake, potentially reducing pathogenicity. Additionally, entC2 serves as a tool in structural biology (e.g., crystallography) to map active sites and design inhibitors.
Current research explores entC2's biotechnological applications, including biosensors for iron bioavailability or chassis engineering for synthetic siderophore production. Despite progress, challenges remain in optimizing expression conditions and understanding regulatory mechanisms in diverse bacterial species. Overall, entC2 recombinant protein is a key molecule in both basic microbiology and applied antimicrobial development.
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