| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ces1c |
| Uniprot No | P23953 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-550aa |
| 氨基酸序列 | HSLLPPVVDTTQGKVLGKYISLEGFEQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSFVKNATSYPPMCSQDAGWAKILSDMFSTEKEILPLKISEDCLYLNIYSPADLTKSSQLPVMVWIHGGGLVIGGASPYNGLALSAHENVVVVTIQYRLGIWGLFSTGDEHSPGNWAHLDQLAALRWVQDNIANFGGNPDSVTIFGESSGGISVSVLVLSPLGKDLFHRAISESGVVINTNVGKKNIQAVNEIIATLSQCNDTSSAAMVQCLRQKTESELLEISGKLVQYNISLSTMIDGVVLPKAPEEILAEKSFNTVPYIVGFNKQEFGWIIPMMLQNLLPEGKMNEETASLLLRRFHSELNISESMIPAVIEQYLRGVDDPAKKSELILDMFGDIFFGIPAVLLSRSLRDAGVSTYMYEFRYRPSFVSDKRPQTVEGDHGDEIFFVFGAPLLKEGASEEETNLSKMVMKFWANFARNGNPNGEGLPHWPEYDEQEGYLQIGATTQQAQRLKAEEVAFWTELLAKNPPETDPTEH |
| 预测分子量 | 63.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ces1c重组蛋白的3篇参考文献及其摘要概览：
---
**1. 文献名称**：*"Expression and Functional Characterization of Recombinant CES1C in Human Cell Lines"*
**作者**：Johnson R., et al.
**摘要**：本研究成功在HEK293细胞中表达了重组CES1C蛋白，并验证其羧酸酯酶活性。通过体外实验证明，CES1C对多种临床药物(如抗血小板药物氯吡格雷)具有显著水解能力，提示其在个体化用药代谢研究中的潜在价值。
---
**2. 文献名称**：*"Structural Determinants of Substrate Specificity in Recombinant CES1C: A Molecular Dynamics Approach"*
**作者**：Chen L., Wang X., et al.
**摘要**：利用重组CES1C蛋白的晶体结构结合分子动力学模拟，揭示了其活性位点对底物立体选择性的关键机制，发现疏水通道的构象变化直接影响酶与底物的结合效率，为理性设计CES1C特异性底物提供理论依据。
---
**3. 文献名称**：*"High-Yield Production of Active Recombinant CES1C in Pichia pastoris for Biocatalytic Applications"*
**作者**：Müller S., et al.
**摘要**：开发了基于毕赤酵母系统的CES1c重组蛋白高效表达策略，优化后蛋白产量达50 mg/L，并证实其催化稳定性适用于工业级酯类前体的生物转化，拓展了其在绿色化学中的应用潜力。
---
**注**：以上文献信息为示例性概括，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“CES1C recombinant protein”进一步检索具体文献。
**Background of CES1C Recombinant Protein**
Carboxylesterase 1C (CES1C), a member of the carboxylesterase enzyme family, plays a critical role in the metabolism of endogenous and exogenous compounds. These enzymes are primarily involved in hydrolyzing ester- and amide-containing molecules, facilitating the detoxification of drugs, environmental toxins, and bioactive lipids. CES1C, encoded by the *CES1* gene cluster in humans, shares structural and functional similarities with other CES isoforms (e.g., CES1A1) but exhibits distinct substrate specificity and tissue expression patterns. It is predominantly expressed in the liver and macrophages, contributing to lipid homeostasis, drug activation (e.g., prodrug conversion), and modulation of inflammatory responses.
Recombinant CES1C protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified enzymes for functional and structural studies. Its recombinant form enables researchers to explore its catalytic mechanisms, substrate preferences, and interactions with inhibitors or activators. This is particularly relevant in pharmaceutical research, as CES1C influences the pharmacokinetics of therapeutics, including antivirals, chemotherapeutics, and lipid-lowering agents. Dysregulation of CES1C has been linked to metabolic disorders, cardiovascular diseases, and cancer, underscoring its therapeutic and diagnostic potential.
Studies leveraging recombinant CES1C also aim to address interindividual variability in drug responses, often attributed to genetic polymorphisms affecting enzyme activity. By characterizing these variants, researchers strive to optimize drug design and personalize treatment strategies. Overall, recombinant CES1C serves as a vital tool for advancing drug development, toxicology, and understanding metabolic pathways in health and disease.
×
