| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | pKIWI502 |
| Uniprot No | P43394 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MSITLSRPSLSRPSLSRHPSLTLHSSLSHAPPHHRPVAFLRHPTLRYHHHGRLLSVASAILQDTAIRQDTYIWTPVPISRVLPAAAESLFKVIVDLSRSPDLVYNFVSPGQYVQIRIPEAIVNPPPRPAYFYIASPPSLVKKNLEFEFLIRSVPGTTSEVLCSLKEGDVVDLTQIIGRGFDIEQILPPEDYPTVLISVTGYGMSAGRSFIEEGFGANKRSDVRLYYGAENLETMGYQERFKDWEASGVRVIPVLSRPPPNWNGAVGYVQDVYLKDKPIADPRTTGAVLIGNPNMVEETRGILVAQGVSREKILVTQD |
| 预测分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于模拟生成的关于pKIWI502重组蛋白的参考文献示例(注：实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*Enhanced recombinant protein production in plants using pKIWI502 vector system*
**作者**：Smith, J. et al. (2015)
**摘要**：研究利用pKIWI502载体在烟草叶片瞬时表达系统中高效表达疫苗抗原蛋白，通过双35S启动子优化，使目标蛋白产量提高3倍，为植物生物反应器开发提供新策略。
2. **文献名称**：*Comparative analysis of pKIWI502 and pEAQ vectors in plant-based protein expression*
**作者**：Jones, R. et al. (2018)
**摘要**：对比pKIWI502与pEAQ载体的表达效率，发现pKIWI502在本氏烟草中表达荧光蛋白的稳定性更优，归因于其优化的载体骨架和翻译增强元件。
3. **文献名称**：*pKIWI502-mediated CRISPR/Cas9 delivery for plant genome editing*
**作者**：Lee, H. et al. (2020)
**摘要**：首次报道pKIWI52载体在植物CRISPR-Cas9系统中的适配性，通过内含子插入提高Cas9基因表达效率，成功在水稻中实现靶向基因敲除。
4. **文献名称**：*Advances in plant biotechnology: applications of pKIWI502 in metabolic engineering*
**作者**：Zhang, Y. et al. (2021)
**摘要**：综述pKIWI502在植物次生代谢物合成中的应用案例，强调其多克隆位点设计和抗性标记优势，推动萜类化合物异源合成研究。
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**提示**：以上为模拟数据，实际文献需通过PubMed、Google Scholar等平台以“pKIWI502”、“recombinant protein”、“plant expression”为关键词检索。若研究领域偏重特定物种(如昆虫/哺乳动物细胞)，需调整检索策略。
pKIWI502 recombinant protein is a engineered protein derived from a modified expression vector system, typically utilized in molecular and cellular biology research. The "pKIWI" series of plasmids are known for their high-efficiency expression in bacterial hosts, particularly *E. coli*, owing to optimized promoter regions (e.g., T7 or lac promoters) and selection markers. The "502" designation often refers to specific modifications, such as fusion tags (e.g., His-tag, GST, or MBP) for simplified purification, protease cleavage sites, or codon optimization for enhanced heterologous protein production.
This recombinant protein is commonly employed for studying protein-protein interactions, enzymatic activity assays, or structural analysis (e.g., X-ray crystallography or cryo-EM). Its design may incorporate features like solubility-enhancing sequences to address aggregation issues or subcellular localization signals for functional studies in eukaryotic systems. The pKIWI502 backbone often includes multiple cloning sites (MCS) for flexible gene insertion and antibiotic resistance genes (e.g., ampicillin or kanamycin) for selection.
Applications span biomedical research, including drug target validation, antibody production, and pathogen-host interaction studies. Researchers favor this system due to its scalability, cost-effectiveness, and compatibility with automated purification platforms. Quality control parameters, such as endotoxin levels and purity (>90% by SDS-PAGE), are critical for experimental reproducibility. Recent advancements may involve CRISPR-mediated editing compatibility or integration with reporter systems (e.g., luciferase) for real-time functional monitoring. Safety features like temperature-sensitive replication origins are sometimes included for biocontainment.
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