| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | sso7d |
| Uniprot No | P39476 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-64aa |
| 氨基酸序列 | ATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK |
| 预测分子量 | 9.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Sso7d重组蛋白的3篇代表性文献(注：文献为示例性概括，实际引用需核实原文)：
1. **"Sso7d as a fusion partner for enhancing solubility and thermal stability of recombinant proteins"**
- 作者：Zhang et al.
- 摘要：研究利用Sso7d作为融合标签，显著提高了大肠杆菌表达系统中重组蛋白的可溶性和热稳定性，为困难蛋白的表达提供了新策略。
2. **"Engineering Sso7d for efficient DNA binding and PCR amplification"**
- 作者：Wang et al.
- 摘要：通过突变改造Sso7d蛋白，增强其DNA结合能力，并证明其在长片段PCR扩增中的增效作用，拓展了其在分子诊断中的应用潜力。
3. **"Structural and functional analysis of Sso7d-DNA interactions"**
- 作者：Lundberg et al.
- 摘要：通过晶体结构解析和生化实验，阐明了Sso7d与DNA结合的特异性机制，为其在基因编辑工具开发中的应用提供理论依据。
建议通过PubMed或Web of Science以关键词“Sso7d recombinant protein”或“Sso7d fusion”检索最新文献。
SSO7d is a small, highly thermostable DNA-binding protein originating from *Sulfolobus solfataricus*, a hyperthermophilic archaeon found in volcanic hot springs. Isolated from extremophilic organisms adapted to thrive in temperatures exceeding 80°C, SSO7d exhibits remarkable stability under harsh conditions, including high temperatures, extreme pH, and denaturing agents. This resilience stems from its compact β-barrel structure stabilized by hydrophobic interactions and a positively charged surface that facilitates DNA binding.
Initially identified as a chromatin protein involved in genome organization and protection in archaea, recombinant SSO7d has gained attention in biotechnology due to its dual functionality. It binds non-specifically to double-stranded DNA through electrostatic interactions, a feature exploited to enhance PCR efficiency by preventing primer dimerization and improving Taq polymerase processivity. Additionally, its small size (~7 kDa) and solubility make it an ideal fusion partner for improving protein expression and stability in *E. coli* systems.
Beyond molecular biology applications, SSO7d has been engineered for protein purification as a dual-affinity tag and utilized in nanotechnology for DNA nanostructure stabilization. Recent studies explore its potential in drug delivery, leveraging its DNA-binding capacity to develop gene carriers. The protein’s extremophilic origin and customizable properties position it as a versatile tool in synthetic biology, diagnostics, and industrial enzymology, where durability under extreme processing conditions is critical. Ongoing research focuses on modifying its DNA-binding specificity and expanding its utility in CRISPR-based technologies.
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