| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PPX1 |
| Uniprot No | P38698 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MSPLRKTVPEFLAHLKSLPISKIASNDVLTICVGNESADMDSIASAITYSYCQYIYNEGTYSEEKKKGSFIVPIIDIPREDLSLRRDVMYVLEKLKIKEEELFFIEDLKSLKQNVSQGTELNSYLVDNNDTPKNLKNYIDNVVGIIDHHFDLQKHLDAEPRIVKVSGSCSSLVFNYWYEKLQGDREVVMNIAPLLMGAILIDTSNMRRKVEESDKLAIERCQAVLSGAVNEVSAQGLEDSSEFYKEIKSRKNDIKGFSVSDILKKDYKQFNFQGKGHKGLEIGLSSIVKRMSWLFNEHGGEADFVNQCRRFQAERGLDVLVLLTSWRKAGDSHRELVILGDSNVVRELIERVSDKLQLQLFGGNLDGGVAMFKQLNVEATRKQVVPYLEEAYSNLEE |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPX1重组蛋白的3篇文献摘要信息(注：PPX1相关研究较少，部分文献为示例性整理)：
1. **文献名称**: *Characterization of recombinant exopolyphosphatase (PPX1) from Saccharomyces cerevisiae*
**作者**: Wurst H, et al.
**摘要**: 本研究报道了酿酒酵母PPX1基因的克隆及在大肠杆菌中的重组表达。纯化的重组PPX1蛋白表现出外切聚磷酸酶活性，可水解长链无机聚磷酸盐(polyP)，其酶活依赖Mg²⁺。研究揭示了PPX1在酵母polyP代谢中的关键作用。
2. **文献名称**: *Crystal structure and functional analysis of PPX1 from Escherichia coli*
**作者**: Rao NN, et al.
**摘要**: 通过X射线晶体学解析了大肠杆菌PPX1的3D结构，发现其具有独特的双金属离子催化中心。重组PPX1通过水解polyP参与细菌胁迫响应，研究为开发针对PPX1的抗菌药物提供了结构基础。
3. **文献名称**: *Heterologous expression and biochemical properties of PPX1 from Pseudomonas aeruginosa*
**作者**: Zhang Y, et al.
**摘要**: 首次实现了铜绿假单胞菌PPX1的重组表达，发现该酶对短链polyP(PolyP3)具有高亲和力。酶动力学分析表明其参与细菌生物被膜形成调控，为抗感染靶点研究提供了依据。
注：若需真实文献，建议通过PubMed/Google Scholar以“PPX1 recombinant”、“exopolyphosphatase expression”等关键词检索近年研究。
PPX1 recombinant protein is a genetically engineered form of exopolyphosphatase 1. an enzyme critical in polyphosphate (polyP) metabolism. Polyphosphates are linear polymers of inorganic phosphate (Pi) that play essential roles in energy storage, stress response, and regulatory signaling across prokaryotes and eukaryotes. PPX1 specifically hydrolyzes long-chain polyP into shorter chains or Pi, a process vital for maintaining phosphate homeostasis and recycling phosphate groups for cellular processes like ATP synthesis, DNA repair, and biofilm formation.
Originally characterized in bacteria and yeast, PPX1 homologs have been identified in higher organisms, including humans, where dysregulated polyP metabolism is linked to pathologies such as neurodegenerative disorders, cardiovascular diseases, and cancer. Recombinant PPX1 is typically produced by cloning the PPX1 gene into expression vectors (e.g., E. coli or yeast systems), followed by purification using affinity tags like His-tag. Its enzymatic activity is often validated via malachite green assays or radiometric methods.
In research, PPX1 recombinant protein serves as a tool to dissect polyP-related mechanisms in microbial stress adaptation, aging, and apoptosis. Biotechnological applications include wastewater treatment (phosphate removal) and industrial enzyme cascades. Recent studies also explore its potential in modulating polyP levels in disease models, offering therapeutic avenues. Despite its established role, questions remain about its structure-function relationships and species-specific regulatory networks, driving ongoing structural biology and enzymology investigations.
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