| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | phyA |
| Uniprot No | P34752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-467aa |
| 氨基酸序列 | ASRNQSSCDTVDQGYQCFSETSHLWGQYAPFFSLANESVISPEVPAGCRVTFAQVLSRHGARYPTDSKGKKYSALIEEIQQNATTFDGKYAFLKTYNYSLGADDLTPFGEQELVNSGIKFYQRYESLTRNIVPFIRSSGSSRVIASGKKFIEGFQSTKLKDPRAQPGQSSPKIDVVISEASSSNNTLDPGTCTVFEDSELADTVEANFTATFVPSIRQRLENDLSGVTLTDTEVTYLMDMCSFDTISTSTVDTKLSPFCDLFTHDEWINYDYLQSLKKYYGHGAGNPLGPTQGVGYANELIARLTHSPVHDDTSSNHTLDSSPATFPLNSTLYADFSHDNGIISILFALGLYNGTKPLSTTTVENITQTDGFSSAWTVPFASRLYVEMMQCQAEQEPLVRVLVNDRVVPLHGCPVDALGRCTRDSFVRGLSFARSGGDWAECFA |
| 预测分子量 | 50.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于phyA重组蛋白的3篇文献示例(内容为模拟，仅供参考)：
1. **《高效表达phyA重组植酸酶的大肠杆菌系统构建》**(Zhang et al., 2015)
摘要：研究通过克隆phyA基因至大肠杆菌表达系统，优化诱导条件后获得高活性重组植酸酶，酶活达5000 U/mg，适用于饲料添加剂。
2. **《毕赤酵母中phyA重组蛋白的分泌表达及热稳定性改造》**(Wang et al., 2018)
摘要：利用毕赤酵母分泌表达phyA，通过定点突变提升酶的热稳定性(70℃处理30分钟保留80%活性)，为工业化生产提供策略。
3. **《重组phyA植酸酶在肉鸡饲料中的磷释放效应研究》**(Liu et al., 2020)
摘要：动物实验表明，添加重组phyA可提高肉鸡磷吸收率25%，减少粪便磷污染，证实其农业应用潜力。
(注：以上文献信息为示例，实际文献需通过数据库查询。)
**Background of PhyA Recombinant Protein**
Phytochrome A (PhyA) is a plant photoreceptor that plays a central role in mediating light-dependent developmental processes, particularly in response to far-red and red light. As a member of the phytochrome family, PhyA exists in two photo-interconvertible forms: the inactive Pr form (absorbing red light, ~660 nm) and the active Pfr form (absorbing far-red light, ~730 nm). This reversible conformational change enables PhyA to act as a biological switch, regulating critical phenomena such as seed germination, shade avoidance, photomorphogenesis, and flowering time. Unlike other phytochromes (e.g., PhyB), PhyA is light-labile and primarily governs responses under low-light conditions or continuous far-red light.
Recombinant PhyA proteins are engineered through heterologous expression systems, such as *E. coli*, yeast, or plant cell cultures, to study its structure-function relationships and signaling mechanisms. The protein comprises two major domains: an N-terminal photosensory module (including a PAS-GAF-PHY architecture for chromophore binding and light sensing) and a C-terminal regulatory region involved in dimerization and interaction with downstream signaling partners. The chromophore, typically phytochromobilin, is covalently attached to the GAF domain, enabling light absorption.
Producing functional recombinant PhyA requires optimizing expression conditions to ensure proper chromophore incorporation and post-translational modifications. Purification often involves affinity tags (e.g., His-tag) and chromatographic techniques. Recombinant PhyA has been instrumental in elucidating light-signaling pathways, including its role in nuclear translocation, interaction with transcription factors (e.g., PIFs), and degradation via the ubiquitin-proteasome system. Additionally, it serves as a tool for biotechnological applications, such as optogenetics and crop engineering to enhance light-use efficiency. Studies using recombinant PhyA continue to advance our understanding of plant adaptation to environmental cues and offer potential strategies for improving agricultural productivity.
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