| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WAXY |
| Uniprot No | P27736 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 79-349aa |
| 氨基酸序列 | LVFVGAEMAPWSKTGGLGDVLGGLPAAMAANGHRVMVISPRYDQYKDAWDTSVISEIKVVDRYERVRYFHCYKRGVDRVFVDHPCFLEKVRGKTKEKIYGPDAGTDYEDNQQRFSLLCQAALEVPRILDLNNNPHFSGPYAMLCRAVPRRAGEDVVFVCNDWHTGLLACYLKSNYQSNGIYRTAKVAFCIHNISYQGRFSFDDFAQLNLPDRFKSSFDFIDGYDKPVEGRKINWMKAGILQADKVLTVSPYYAEELISGEARGCELDNIMR |
| 预测分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WAXY重组蛋白的3篇参考文献示例(内容基于学术研究背景虚构，供参考)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant WAXY Protein in Transgenic Rice*
**作者**：T. Nakamura, Y. Sato, et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组水稻WAXY蛋白，并验证其淀粉颗粒结合活性。通过体外酶活实验证实，重组蛋白可催化直链淀粉合成，为改良作物淀粉品质提供理论依据。
2. **文献名称**：*Structural Characterization of WAXY Recombinant Protein in Wheat*
**作者**：M. Morell, R. Appels
**摘要**：通过X射线晶体学解析小麦重组WAXY蛋白的三维结构，发现其N端结构域对淀粉合酶活性至关重要。研究揭示了该蛋白在淀粉分支中的分子机制，为设计高直链淀粉作物奠定基础。
3. **文献名称**：*Heterologous Expression of Maize WAXY Gene in Yeast for Industrial Applications*
**作者**：J. Li, C. Zhang
**摘要**：将玉米WAXY基因导入毕赤酵母进行重组表达，优化发酵条件后蛋白产量提高3倍。重组产物在食品工业中展示出高效催化直链淀粉生成的能力，具有潜在工业化生产价值。
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以上文献方向涵盖重组表达系统、结构功能解析及工业应用，符合WAXY蛋白研究的核心领域。如需真实文献，建议在PubMed或Web of Science中检索关键词“Waxy protein recombinant expression”。
**Background of WAXY Recombinant Proteins**
WAXY recombinant proteins represent a class of engineered proteins designed to address challenges in biomanufacturing, therapeutic development, and industrial enzymology. The term "WAXY" derives from their unique structural or functional attributes, often linked to wax-like properties, enhanced stability, or modularity. These proteins are synthesized using recombinant DNA technology, where target genes are cloned into expression vectors and expressed in host systems like *E. coli*, yeast, or mammalian cells.
The development of WAXY proteins is driven by the need for customizable biomolecules with tailored functionalities. For instance, in therapeutics, they may serve as scaffolds for antibody mimics, drug carriers, or bioactive molecules with improved pharmacokinetics. Industrial applications leverage their robustness in extreme conditions (e.g., high temperature, pH variability) for processes like biofuel production or waste degradation.
A key innovation in WAXY protein design is the integration of computational modeling and directed evolution. These approaches optimize protein folding, ligand binding, or catalytic efficiency, enabling precise customization. Additionally, advancements in synthetic biology have facilitated the incorporation of non-canonical amino acids or fusion tags (e.g., His-tags, fluorescent markers) to enhance purification or tracking.
Despite their potential, challenges persist, including scalability, immunogenicity in therapeutic contexts, and cost-effective production. Current research focuses on refining expression systems (e.g., plant-based platforms) and minimizing post-translational modifications that may affect functionality. WAXY recombinant proteins exemplify the convergence of bioengineering and biotechnology, offering versatile solutions across healthcare, agriculture, and sustainable industries. Their continued evolution aligns with global demands for precision biotech tools and environmentally friendly alternatives.
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