| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ctfB |
| Uniprot No | P23673 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-221aa |
| 氨基酸序列 | MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFKITFQSENGIVGMGASPKINEADKDVVNAGGDYTTVLPDGTFFDSSVSFSLIRGGHVDVTVLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKKCTLPLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV |
| 预测分子量 | 30.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ctfB重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**：*Functional expression of the Clostridium acetobutylicum acetoacetate decarboxylase gene in Escherichia coli*
**作者**：Petersen DJ, Bennett GN
**摘要**：该研究成功将产酸梭菌的ctfB基因(编码乙酰乙酸脱羧酶)在大肠杆菌中重组表达，证实了重组蛋白的酶活性，并分析了其在丙酮-丁醇发酵途径中的作用。
2. **文献名称**：*Purification and characterization of recombinant CoA transferase (CtfB) from Clostridium acetobutylicum*
**作者**：Hartmanis MG, Klason T
**摘要**：报道了ctfB编码的辅酶A转移酶(CoA transferase)在大肠杆菌中的重组表达与纯化，通过酶动力学分析揭示了其在丁酸代谢中的催化机制。
3. **文献名称**：*Metabolic engineering of Clostridium acetobutylicum with overexpressed ctfB for enhanced butanol production*
**作者**：Jiang Y, Xu C et al.
**摘要**：通过过表达重组ctfB蛋白优化产酸梭菌的丁醇合成途径，实验表明重组菌株的溶剂产量显著提高，为生物燃料生产提供了新策略。
注：以上文献为示例，实际引用时建议通过PubMed或Web of Science核实具体信息。ctfB相关研究多聚焦于梭菌的溶剂生成代谢调控及酶工程应用。
**Background of ctfB Recombinant Protein**
The **ctfB** gene, derived from solventogenic clostridia such as *Clostridium acetobutylicum*, encodes a critical enzyme involved in the acetone-butanol-ethanol (ABE) fermentation pathway. This enzyme, known as CoA-transferase (CtfB), works in tandem with CtfA to catalyze the conversion of acetoacetyl-CoA into acetoacetate, a key step in solventogenesis. CtfB is essential for redirecting metabolic flux toward acetone production, making it a target for metabolic engineering in biofuel research.
Recombinant CtfB protein is typically produced via heterologous expression in hosts like *E. coli*, enabling scalable purification and functional studies. Its recombinant form retains enzymatic activity, allowing researchers to explore its structure, kinetics, and interactions with substrates or inhibitors. Studies have highlighted its role in enhancing solvent yields when overexpressed in clostridial strains or engineered microbial systems.
Interest in CtfB stems from its biotechnological potential in sustainable biofuel production, particularly for butanol, a promising renewable energy source. Additionally, structural analyses of recombinant CtfB have provided insights into its mechanism, including its dependence on CoA-thioester substrates and its dimeric configuration for activity. Challenges remain in optimizing its stability and activity in non-native hosts, driving ongoing research into protein engineering and fermentation process design.
Overall, CtfB recombinant protein serves as a valuable tool for both fundamental studies of clostridial metabolism and applied efforts to advance biofuel technologies.
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