| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AAP1 |
| Uniprot No | P37898 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MSREVLPNNVTPLHYDITLEPNFRAFTFEGSLKIDLQINDHSINSVQINYLEIDFHSARIEGVNAIEVNKNENQQKATLVFPNGTFENLGPSAKLEIIFSGILNDQMAGFYRAKYTDKVTGETKYMATTQMEATDARRAFPCFDEPNLKATFAVTLVSESFLTHLSNMDVRNETIKEGKKYTTFNTTPKMSTYLVAFIVADLRYVESNNFRIPVRVYSTPGDEKFGQFAANLAARTLRFFEDTFNIEYPLPKMDMVAVHEFSAGAMENWGLVTYRVIDLLLDIENSSLDRIQRVAEVIQHELAHQWFGNLVTMDWWEGLWLNEGFATWMSWYSCNKFQPEWKVWEQYVTDNLQRALNLDSLRSSHPIEVPVNNADEINQIFDAISYSKG |
| 预测分子量 | 60.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AAP1重组蛋白的3篇模拟参考文献(注：部分信息为假设性示例，实际文献需通过数据库核实)：
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1. **文献名称**：Functional Characterization of Arabidopsis AAP1 Recombinant Protein in Amino Acid Transport
**作者**：Hirner, B., et al.
**摘要**：本研究通过原核系统表达拟南芥AAP1重组蛋白，验证其作为氨基酸通透酶的活性。实验表明AAP1在体外可高效转运中性氨基酸，并揭示了其在植物根部氨基酸长距离运输中的关键作用。
2. **文献名称**：Heterologous Expression of AAP1 in Yeast: A Model for Plant Amino Acid Transporters
**作者**：Fischer, W.N., et al.
**摘要**：作者在酵母中重组表达AAP1蛋白，通过互补实验证明其恢复酵母突变体的氨基酸吸收能力。研究提供了AAP1底物特异性及动力学参数，为植物氨基酸转运机制提供了分子证据。
3. **文献名称**：Crystal Structure and Biochemical Analysis of Recombinant AAP1 from Oryza sativa
**作者**：Lee, S., & Kim, J.
**摘要**：该研究解析了水稻AAP1重组蛋白的晶体结构，结合定点突变实验，揭示了跨膜结构域中关键氨基酸残基对底物识别和转运的调控机制，为设计改良作物氮利用效率提供理论依据。
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如需真实文献，建议在PubMed或Web of Science中搜索关键词“AAP1 recombinant protein”或结合具体物种名称(如拟南芥、水稻等)进行筛选。
**Background of AAP1 Recombinant Protein**
AAP1 (amino acid permease 1) is a membrane transport protein primarily studied in plants, fungi, and microorganisms for its role in amino acid uptake and homeostasis. It belongs to the amino acid/auxin permease (AAAP) family, which facilitates the transport of amino acids across cellular membranes. In plants, such as *Arabidopsis thaliana*, AAP1 is localized to the plasma membrane and is critical for root uptake of neutral and acidic amino acids from the soil, influencing nitrogen distribution, seed development, and stress responses. Dysregulation of AAP1 has been linked to altered growth phenotypes and metabolic imbalances, highlighting its importance in nutrient management.
Recombinant AAP1 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells) to enable detailed biochemical and structural studies. By cloning the *AAP1* gene into expression vectors, researchers produce purified AAP1 to analyze its transport kinetics, substrate specificity, and regulatory mechanisms. This approach bypasses challenges associated with isolating native AAP1 from plant tissues, ensuring higher yields and purity.
Studies on recombinant AAP1 have clarified its role in nutrient signaling and potential applications in agriculture, such as engineering crops with enhanced nitrogen-use efficiency. Additionally, AAP1 homologs in pathogens (e.g., *Candida albicans*) are explored as drug targets, as amino acid transporters are vital for microbial virulence.
Overall, AAP1 recombinant protein serves as a key tool for unraveling amino acid transport biology, with implications for crop improvement, antimicrobial therapies, and understanding metabolic disorders.
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