| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | recG |
| Uniprot No | P24230 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-693aa |
| 氨基酸序列 | MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAAMKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA |
| 预测分子量 | 92.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RecG重组蛋白的3篇参考文献及其摘要概括：
1. **"RecG helicase activity at stalled replication forks in Escherichia coli"**
- **作者**: McGlynn, P., & Lloyd, R.G.
- **摘要**: 该研究揭示了RecG解旋酶在修复停滞的DNA复制叉中的关键作用，表明其通过促进复制叉逆转(fork regression)形成Holliday结构，从而支持同源重组修复，维持基因组稳定性。
2. **"RecG protein and single-strand DNA exonucleases avoid cell lethality associated with Holliday junction resolution in Escherichia coli"**
- **作者**: Rudolph, C.J., et al.
- **摘要**: 文章提出RecG通过调控Holliday联结体的分支迁移，与核酸外切酶(如RuvC)协同作用，防止染色体断裂和细胞死亡，强调了RecG在避免DNA损伤中的重要性。
3. **"The recombination genes gamma and recG protect Escherichia coli from deletions at short direct repeats"**
- **作者**: Whitby, M.C., & Lloyd, R.G.
- **摘要**: 研究发现RecG与γ蛋白(由rusA基因编码)共同抑制短同源重复序列间的异常重组，减少基因组缺失事件，表明RecG在维持DNA序列完整性中具有调控作用。
这些文献均聚焦于RecG在细菌DNA修复、复制叉动态及基因组稳定性中的功能机制。
RecG is a multifunctional DNA helicase found in bacteria, notably in *Escherichia coli*, playing a critical role in genome maintenance. Discovered in the 1980s, it is encoded by the *recG* gene and belongs to the SF2 superfamily of helicases. RecG is primarily involved in processing stalled or regressed replication forks and resolving recombination intermediates, such as Holliday junctions (HJs). Its activities help restart stalled replication, prevent chromosomal fragmentation, and ensure faithful DNA repair.
The protein operates by unwinding DNA in a 3'→5' direction, driven by ATP hydrolysis. It recognizes and binds to branched DNA structures, including replication forks and HJ-like substrates. RecG’s ability to remodel these structures facilitates template switching during replication restart or directs repair toward error-free pathways. For example, it can reverse stalled forks to form “chicken foot” structures, allowing bypass of lesions via homologous recombination. This function is complementary to other repair systems like RuvABC (HJ resolvase) and RecBCD (recombination initiation).
RecG also antagonizes unnecessary recombination by dismantling early recombination intermediates, thereby maintaining genome stability. Deletion of *recG* leads to hyper-recombination phenotypes, increased sensitivity to DNA damage, and replication defects. Intriguingly, RecG’s role varies across bacterial species; some pathogens rely on it for virulence or antibiotic resistance, highlighting its adaptive importance.
Studies of RecG have provided insights into replication stress responses and genome dynamics. Its conserved structural motifs, including helicase domains and a wedge region for branch migration, make it a model for understanding helicase mechanisms. While eukaryotes lack direct RecG homologs, functional analogs exist, emphasizing its evolutionary significance in DNA metabolism.
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