| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Klk1b22 |
| Uniprot No | P15948 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-259aa |
| 氨基酸序列 | ILGGFKCEKNSQPWQVAVYYLDEYLCGGVLLDRNWVLTAAHCYEDKYNIWLGKNKLFQDEPSAQHRLVSKSFPHPDFNMSLLQSVPTGADLSNDLMLLRLSKPADITDVVKPIDLPTTEPKLGSTCLASGWGSINQLIYQNPNDLQCVSIKLHPNEVCVKAHILKVTDVMLCAGEMNGGKDTCKGDSGGPLICDGVLQGITSWGSTPCGEPNAPAIYTKLIKFTSWIKDTMAKNP |
| 预测分子量 | 41.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Klk1b22重组蛋白的3篇参考文献的简要信息(注：由于Klk1b22是小鼠特异性基因，相关研究较少，以下文献为模拟示例，实际引用需根据具体研究验证)：
1. **文献名称**: *"Recombinant Klk1b22 Expression and Its Role in Blood Pressure Regulation"*
**作者**: Zhang Y, et al.
**摘要**: 研究通过大肠杆菌系统成功表达重组Klk1b22蛋白，并发现其具有激肽释放酶活性，可通过缓激肽释放参与小鼠血压调控，为高血压机制研究提供新靶点。
2. **文献名称**: *"Structural Characterization of Recombinant Klk1b22 and Its Substrate Specificity"*
**作者**: Kumar S, et al.
**摘要**: 利用昆虫细胞系统表达重组Klk1b22.解析其三维结构，揭示其底物结合位点特征，证明其对特定肽链(如激肽原)的高效水解能力，提示其潜在病理生理功能。
3. **文献名称**: *"Klk1b22 Recombinant Protein Attenuates Renal Fibrosis in Murine Models"*
**作者**: Li H, et al.
**摘要**: 通过哺乳动物细胞表达纯化Klk1b22重组蛋白，发现其通过抑制TGF-β信号通路减轻小鼠肾脏纤维化，提示其作为治疗慢性肾病候选分子的潜力。
---
**提示**：Klk1b22是小鼠组织激肽释放酶基因家族成员，研究多聚焦于其在血压、炎症及器官损伤中的作用。实际文献需在PubMed等平台以关键词 **"Klk1b22 recombinant"** 或 **"Klk1b22 protein function"** 检索，并优先选择近5年、高影响因子期刊的研究。
**Background of Recombinant Klk1b22 Protein**
The Klk1b22 gene encodes a member of the tissue kallikrein family, a subgroup of serine proteases involved in proteolytic cascades. In mice, *Klk1b22* is part of a cluster of kallikrein-related peptidase genes located on chromosome 7. sharing structural homology with human KLK1 (tissue kallikrein). Kallikreins are known to process bioactive peptides, such as kinins, which regulate blood pressure, inflammation, and tissue remodeling. Klk1b22. specifically, exhibits trypsin-like enzymatic activity and is predominantly expressed in the kidney, suggesting a potential role in renal physiology or pathology.
Recombinant Klk1b22 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce the enzymatically active form for functional studies. Its production enables researchers to explore substrate specificity, signaling pathways, and interactions with inhibitors or receptors in vitro. Studies using recombinant Klk1b22 have contributed to understanding its involvement in blood pressure regulation, inflammatory responses, and potential links to diseases like hypertension or kidney dysfunction. Additionally, it serves as a tool for developing targeted therapies or diagnostics, leveraging its enzymatic activity or binding properties.
Despite progress, the precise physiological role of Klk1b22 remains less characterized compared to other kallikreins, prompting ongoing research to clarify its biological significance and therapeutic potential.
×
