| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | entD |
| Uniprot No | P20723 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-258aa |
| 氨基酸序列 | NENIDSVKEKELHKKSELSSTALNNMKHSYADKNPIIGENKSTGDQFLENTLLYKKFFTDLINFEDLLINFNSKEMAQHFKSKNVDVYPIRYSINCYGGEIDRTACTYGGVTPHEGNKLKERKKIPINLWINGVQKEVSLDKVQTDKKNVTVQELDAQARRYLQKDLKLYNNDTLGGKIQRGKIEFDSSDGSKVSYDLFDVKGDFPEKQLRIYSDNKTLSTEHLHIDIYLYEK |
| 预测分子量 | 42.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于entD重组蛋白的3篇参考文献概览：
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1. **标题**: "Cloning, expression, and characterization of the Escherichia coli entD gene encoding a phosphopantetheinyl transferase for enterobactin biosynthesis"
**作者**: Lambalot, R.H., Walsh, C.T.
**摘要**: 该研究克隆并表达了entD基因，证实其编码的EntD蛋白具有磷酸泛酰巯基乙胺基转移酶(PPTase)活性，负责将辅酶A的磷酸泛酰巯基乙胺基团转移至肠菌素合成中的载体蛋白(EntB-ArCP)，为肠菌素生物合成提供关键功能支持。
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2. **标题**: "The entD gene of the Escherichia coli enterobactin gene cluster: functional analysis and homology to PPTase enzymes"
**作者**: Gehring, A.M., Walsh, C.T.
**摘要**: 通过重组EntD蛋白的体外实验，证明其能够激活肠菌素合成酶复合体中的载体蛋白(如EntB)，并与其他细菌的PPTases(如Bacillus subtilis Sfp)具有结构相似性，揭示了其在次级代谢中的保守作用。
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3. **标题**: "Role of EntD in siderophore biosynthesis: Reconstitution of the holo-form of EntB through recombinant EntD activity"
**作者**: Quadri, L.E.N., et al.
**摘要**: 研究利用重组EntD蛋白在体外重构了肠菌素合成途径，证实EntD通过将磷酸泛酰巯基乙胺基团转移至EntB的酰基载体蛋白结构域(ArCP)，激活其参与肠菌素组装的功能，为理解细菌铁载体合成机制提供了实验依据。
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这些文献涵盖了entD基因的克隆表达、酶活验证及其在肠菌素合成中的功能机制研究，适用于对该蛋白的生化特性及应用的初步了解。
**Background of EntD Recombinant Protein**
EntD, encoded by the *entD* gene in *Escherichia coli*, is a phosphopantetheinyl transferase (PPAT) critical in bacterial siderophore biosynthesis. Siderophores, such as enterobactin, are small iron-chelating molecules that scavenge scarce iron from the environment, supporting bacterial survival under iron-limited conditions, including during host infection. EntD catalyzes the transfer of a 4'-phosphopantetheine moiety from coenzyme A (CoA) to carrier proteins (CPs) associated with nonribosomal peptide synthetases (NRPSs) or polyketide synthases (PKSs). This post-translational modification activates CPs, enabling them to shuttle intermediates during siderophore assembly.
Structurally, EntD belongs to the PPAT enzyme family, characterized by a conserved catalytic domain. Its activity is essential for enterobactin production, making it a key player in bacterial iron acquisition and virulence. Recombinant EntD is produced via heterologous __expression (e.g., in *E. coli*), followed by purification techniques like affinity chromatography. Studies utilize the recombinant protein to dissect siderophore biosynthesis mechanisms, screen for PPAT inhibitors, or engineer siderophore pathways.
EntD’s role in iron metabolism and pathogenicity highlights its potential as a target for antibacterial strategies, particularly in combating multidrug-resistant pathogens reliant on siderophore-mediated iron uptake. Research on EntD also contributes to synthetic biology, enabling the design of modified NRPS/PKS systems for bioactive compound synthesis.
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