| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | AC1 |
| Uniprot No | P14982 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MRTPRFRIQAKNVFLTYPKCSIPKEHLLSFIQTLSLQSNPKFIKICRELHQNGEPHLHALIQFEGKITITNNRLFDCVHPSCSTSFHPNIQGAKSSSDVKSYLDKDGDTVEWGQFQIDGRSARGGQQSANDAYAKALNSGSKSEALNVIRELVPKDFVLQFHNLNSNLDRIFQEPPAPYVSPFPCSSFDQVPVEIEEWVADNVRDSAARPWRPNSIVIEGDSRTGKTIWARSLGPHNYLCGHLDLSPKVFNNAAWYNVIDDVDPHYLKHFKEFMGSQRDWQSNTKYGKPVQIKGGIPTIFLCNPGPTSSYKEFLAEEKQEALKAWALKNAIFITLTEPLYSGSNQSHSQTSQEASHPA |
| 预测分子量 | 42.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AC1重组蛋白的模拟参考文献示例(内容为假设性概括，非真实文献)：
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1. **"Expression and Functional Characterization of Recombinant AC1 in Mammalian Cells"**
*Author: Zhang L, et al.*
摘要：研究通过哺乳动物表达系统成功表达AC1重组蛋白，验证其腺苷酸环化酶活性，并证明其参与cAMP信号通路的调控，为神经信号传导研究提供工具。
2. **"Structural Insights into AC1 Activation by G-protein Coupling"**
*Author: Thompson R, et al.*
摘要：利用冷冻电镜解析AC1重组蛋白与Gαs蛋白复合物的三维结构，揭示其激活机制，阐明钙离子与ATP结合位点对酶活性的影响。
3. **"AC1 Recombinant Protein as a Therapeutic Target for Chronic Pain"**
*Author: Kim S, et al.*
摘要：在小鼠模型中证实AC1重组蛋白抑制剂可降低脊髓背角的cAMP水平，显著缓解神经病理性疼痛，提出AC1作为镇痛药物开发的新靶点。
4. **"Optimization of AC1 Recombinant Production in E. coli for High-throughput Screening"**
*Author: Gupta P, et al.*
摘要：开发大肠杆菌中高效可溶表达AC1重组蛋白的工艺，通过亲和层析纯化获得高活性蛋白，用于大规模药物筛选及酶动力学研究。
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注：以上为基于领域知识的模拟文献，实际文献需通过PubMed或Web of Science等数据库检索。若需真实文献，建议以“Adenylyl Cyclase 1 recombinant”或“AC1 protein purification”为关键词查询。
AC1 (adenylate cyclase 1), also known as ADCY1. is a membrane-associated enzyme that catalyzes the conversion of adenosine triphosphate (ATP) into cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. Belonging to the adenylate cyclase family, AC1 is classified as a calcium/calmodulin-stimulated isoform, primarily expressed in neuronal tissues, particularly the brain. Its activity is tightly regulated by intracellular calcium levels and G-protein-coupled receptors (GPCRs), linking extracellular signals to cAMP-dependent pathways involved in synaptic plasticity, memory formation, and neuronal development.
Recombinant AC1 protein is engineered using genetic cloning techniques, often expressed in heterologous systems like insect or mammalian cells to ensure proper post-translational modifications and functional activity. This purified form allows researchers to study AC1's structural properties, enzymatic kinetics, and interactions with regulatory molecules (e.g., calmodulin, Gα subunits) in vitro. AC1 dysregulation has been implicated in neurological disorders, including Alzheimer’s disease and chronic pain syndromes, making it a target for therapeutic intervention. Recombinant AC1 facilitates drug screening for modulators that could restore cAMP homeostasis or selectively inhibit hyperactivity in pathological conditions.
Additionally, AC1's role in long-term potentiation (LTP) and its sensitivity to calcium oscillations highlight its importance in neuroadaptation. Studies using recombinant AC1 have clarified its dual regulation by stimulatory Gαs and inhibitory Gαi/o proteins, as well as its crosstalk with other signaling cascades. Its recombinant form remains a vital tool for dissecting cAMP-driven processes in neuroscience and developing precision therapies for brain-related diseases.
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