| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UL111A |
| Uniprot No | F5HC71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-176aa |
| 氨基酸序列 | ATTTTIKNTKPQCRPEDYATRLQDLRVTFHRVKPTLQREDDYSVWLDGTVVKGCWGCSVMDWLLRRYLEIVFPAGDHVYPGLKTELHSMRSTLESIYKDMRQCPLLGCGDKSVISRLSQEAERKSDNGTRKGLSELDTLFSRLEEYLHSRK |
| 预测分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HCMV UL111A重组蛋白的3篇示例参考文献(注：文献为示例性质，具体内容需根据真实文献调整)：
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1. **文献名称**：*"Characterization of HCMV UL111A recombinant protein as a viral interleukin-10 homolog"*
**作者**：Spencer JV, et al.
**摘要**：本研究利用重组技术表达了HCMV UL111A蛋白，证实其与人类IL-10具有高度同源性，并证明其通过抑制促炎细胞因子(如IFN-γ和TNF-α)调控宿主免疫反应，提示其在病毒免疫逃逸中的关键作用。
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2. **文献名称**：*"Structural and functional analysis of recombinant UL111A protein in HCMV latency"*
**作者**：Lee SH, et al.
**摘要**：通过晶体结构解析发现UL111A重组蛋白与宿主IL-10受体结合，并揭示其通过STAT3信号通路维持病毒潜伏感染的分子机制，为靶向UL111A的抗病毒策略提供依据。
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3. **文献名称**：*"Recombinant UL111A protein as a potential vaccine candidate for HCMV infection"*
**作者**：Smith C, et al.
**摘要**：在小鼠模型中评估UL111A重组蛋白的免疫原性，结果显示其可诱导中和抗体及T细胞应答，提示其作为巨细胞病毒疫苗组分的潜在应用价值。
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**备注**：实际文献需通过PubMed、Web of Science等平台检索关键词(如“HCMV UL111A recombinant”)获取。
**Background of UL111A Recombinant Protein**
UL111A is a gene encoded by human cytomegalovirus (HCMV), a betaherpesvirus that establishes lifelong latency in immunocompetent hosts and poses significant risks to immunocompromised individuals or neonates. The UL111A gene product is a viral homolog of human interleukin-10 (hIL-10), termed cmvIL-10. which shares partial functional and structural similarities with its human counterpart. HCMV leverages cmvIL-10 to modulate host immune responses, promoting viral persistence and immune evasion. This protein interacts with the IL-10 receptor (IL-10R), suppressing pro-inflammatory cytokine production, inhibiting antigen presentation, and dampening T-cell responses, thereby facilitating viral survival.
Recombinant UL111A protein is engineered through molecular cloning and expression systems (e.g., mammalian or bacterial cells) to study its immunomodulatory mechanisms. Unlike hIL-10. cmvIL-10 exhibits unique properties, such as preferential binding to the IL-10Rβ subunit and altered signaling outcomes. Research on recombinant UL111A has clarified its role in HCMV pathogenesis, including contributions to viral latency, reactivation, and tissue-specific tropism. Additionally, it serves as a tool for exploring IL-10-related pathways in autoimmune diseases and cancer, where IL-10 signaling is dysregulated.
Studies using UL111A recombinant protein have also informed therapeutic strategies, such as blocking its interaction with host receptors to counteract immune suppression. However, its functional complexity—balancing immune evasion with potential host damage—underscores the need for nuanced approaches in targeting HCMV or IL-10 pathways. Overall, UL111A recombinant protein remains a critical reagent for unraveling HCMV biology and IL-10-mediated immune regulation.
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