| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UL128 |
| Uniprot No | P16837 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-171aa |
| 氨基酸序列 | MSPKDLTPFLTTLWLLLGHSRVPRVRAEECCEFINVNHPPERCYDFKMCNRFTVALRCPDGEVCYSPEKTAEIRGIVTTMTHSLTRQVVHNKLTSCNYNPLYLEADGRIRCGKVNDKAQYLLGAAGSVPYRWINLEYDKITRIVGLDQYLESVKKHKRLDVCRAKMGYMLQ |
| 预测分子量 | 23.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UL128重组蛋白的示例性参考文献(模拟内容，供参考)：
1. **文献名称**：Expression and Purification of HCMV UL128 Recombinant Protein in Mammalian Cells
**作者**：Smith A, et al.
**摘要**：研究利用哺乳动物表达系统成功表达并纯化HCMV UL128重组蛋白，验证其与gH/gL复合体结合的能力，为病毒进入机制研究提供工具。
2. **文献名称**：Structural Characterization of UL128 in Human Cytomegalovirus Entry Pathway
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析UL128重组蛋白与受体结合的结构，阐明其在HCMV感染上皮细胞中的关键作用，为抗病毒药物设计提供依据。
3. **文献名称**：Immunogenicity of UL128 Subunit Vaccine in Animal Models
**作者**：Wang X, et al.
**摘要**：评估UL128重组蛋白作为亚单位疫苗的免疫原性，显示其可诱导中和抗体，降低小鼠模型中的病毒载量，提示疫苗开发潜力。
4. **文献名称**：Optimization of UL128 Expression in E. coli for Antigen Production
**作者**：Zhang Y, et al.
**摘要**：优化大肠杆菌表达系统以提高UL128重组蛋白产量，并证明其用于ELISA诊断试剂的可行性。
**注意**：以上为模拟文献，真实文献建议通过PubMed、Google Scholar或Web of Science搜索关键词“UL128 recombinant protein”“HCMV UL128”等获取。
UL128 recombinant protein is a key component of the human cytomegalovirus (HCMV), a beta-herpesvirus that establishes lifelong latency and causes severe complications in immunocompromised individuals and neonates. The UL128 protein is part of the HCMV Pentamer complex (PC), which also includes UL130. UL131A, glycoproteins gH, and gL. This complex is critical for viral entry into epithelial, endothelial, and myeloid cells, distinguishing it from the gH/gL/gO trimer that mediates fibroblast tropism. UL128 contributes to viral tropism and cell-specific entry mechanisms, making it a focal point for understanding HCMV pathogenesis and host-cell interactions.
Recombinant UL128 is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications and structural fidelity. It is often co-expressed with other PC components to form functional complexes for biochemical and immunological studies. The protein is commonly purified using affinity tags (e.g., His-tag) and characterized via SDS-PAGE, western blotting, and functional assays like neutralization tests. Researchers leverage UL128 recombinant proteins to study HCMV entry mechanisms, screen neutralizing antibodies, and evaluate vaccine candidates targeting non-fibroblast cell types.
Clinically, UL128 has garnered attention due to its immunogenicity. Antibodies against UL128 are associated with reduced vertical transmission of HCMV, positioning it as a potential vaccine target. However, challenges persist in maintaining the conformational integrity of recombinant UL128. as its antigenic properties depend on proper folding within the Pentamer complex. Current research explores its role in viral evasion, cell-to-cell spread, and as a biomarker for HCMV-associated diseases. These efforts aim to develop therapeutic strategies that disrupt viral entry or enhance immune recognition, particularly in high-risk populations.
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