| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ureA |
| Uniprot No | O13465 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-833aa |
| 氨基酸序列 | MHLLPRETDKLILTTLGTLAQRRLARGLILNRAETIALISSQLQEFVRDGRHSVAELMDLGKKMLGRRHVRKGVPESIHTIQVEGTFPDGVFLVTVDDPISSDDGDLNNAFYGSFLPIPSADVFPAAPEPADTLLGALICRKETVKINASRRRFRLEVKNAGDRPVQVGSHYHFLETNPALIFDRLLSYGYHLDIPAGTAVRFEPGEKKTVTMVEFGGKKIFHGGSGLGNGSFDENLRETKVKEMVEKGGFGHKEQEKIEEGPVTEMNREVYASMFGPTTGDKIKLADMDLWIEVEKDYTVYGDECKFGGGKVIRDGGGQASGRHDHEVLDLVITNALIVDWTGIYKADIGVKNGIIVGIGKAGNPDMMDGVTDGMIVGSSTEVISGEKLITTAGALDVHVHYISPQLMTEALASGITTVIGGGTGPADGSNATTCTSSSFYMQNMIKATDTIPLNFGFTGKGSDSGTNAMRDIIEAGACGLKVHEDWGATPEVIDRALSMADEYDVQINLHSDTLNESGYVESTLAAIKGRTIHSYHTEGAGGGHAPDIIVVCEYENVLPSSTNPTRPYAVNTLDEHLDMLMICHGLDKSIPEDIAFADSRIRSETVAAEDVLQDTGAISMISSDCQAMGRIGEVVTRTWRTAAKMKQFRGPLEGDEPTRDNNRVKRYVAKYTINPAITHGMSHLIGQVAVGCLADLVFWTAESFGARPEMILKGGVIAWAAVGDANASIPTVQPVLGRPMWGSQPEAAALNSIVWVSQASLDKDLVKRFNIKKRAEAVKNCRSIGKKDMKWNDTMPKMTVDPETYDVRADGVLCDVPPADKLPLTRRYFVY |
| 预测分子量 | 90,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ureA重组蛋白的3篇参考文献示例(内容为模拟，仅供参考)：
1. **文献名称**: *Cloning and Expression of Helicobacter pylori ureA Gene in E. coli*
**作者**: Smith, J. et al.
**摘要**: 研究利用大肠杆菌系统成功表达幽门螺杆菌ureA基因，获得高纯度重组蛋白，并证实其具备尿素酶活性，为后续疫苗开发奠定基础。
2. **文献名称**: *Immunogenicity of Recombinant UreA Subunit in Mouse Models*
**作者**: Lee, H. & Chen, W.
**摘要**: 通过动物实验证明重组UreA蛋白能诱导强烈的Th1免疫反应，显著降低幽门螺杆菌感染负荷，提示其作为疫苗候选的潜力。
3. **文献名称**: *Structural Analysis of UreA Reveals Key Active Sites*
**作者**: Tanaka, K. et al.
**摘要**: 采用X射线晶体学解析重组UreA蛋白三维结构，发现其镍离子结合位点对脲酶活性至关重要，为开发特异性抑制剂提供结构依据。
*注：以上为模拟文献，实际引用需查询PubMed/Google Scholar等数据库。*
**Background of UreA Recombinant Protein**
UreA is a subunit of the urease enzyme complex, which plays a critical role in nitrogen metabolism by catalyzing the hydrolysis of urea into ammonia and carbon dioxide. This reaction is vital for pathogens like *Helicobacter pylori* to survive in acidic environments, such as the human stomach, by neutralizing gastric acidity. The urease complex typically consists of structural (UreA and UreB) and accessory subunits, with UreA forming the α-subunit that contributes to the enzyme’s stability and catalytic activity.
Recombinant UreA protein is produced through genetic engineering, where the *ureA* gene is cloned into expression vectors (e.g., plasmid systems) and expressed in heterologous hosts like *Escherichia coli*. This approach allows large-scale production of the protein without purifying it directly from pathogenic organisms, enhancing safety and scalability. The recombinant protein is often purified using affinity tags (e.g., His-tag) and chromatography techniques, ensuring high purity for research or diagnostic applications.
Studies on UreA focus on its role in microbial pathogenesis, particularly in *H. pylori*-associated diseases (e.g., gastritis, ulcers, and gastric cancer). It serves as a target for vaccine development and therapeutic interventions, as inhibiting urease activity disrupts bacterial survival. Additionally, recombinant UreA is utilized in serological diagnostics to detect *H. pylori* infections via antibody responses. Structural analyses of UreA, including crystallography and mutagenesis, provide insights into urease assembly and mechanism, guiding drug design.
The use of recombinant UreA also addresses challenges in studying native urease, such as low yield or contamination risks. Its application extends to environmental microbiology, where urease activity influences nitrogen cycling in ecosystems. Overall, UreA recombinant protein is a pivotal tool in understanding bacterial adaptation, developing biomedical solutions, and exploring ecological interactions.
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