| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | nadB |
| Uniprot No | P10902 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540aa |
| 氨基酸序列 | MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEGSTFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGVLFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERSNAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPELLTHSGPSILSPGNHYINR |
| 预测分子量 | 67.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于nadB重组蛋白的3-4篇参考文献的示例(注：以下内容为模拟生成的示例，非真实文献)：
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1. **文献名称**: *Cloning, Expression, and Characterization of Recombinant nadB Gene Encoding L-Aspartate Oxidase in Escherichia coli*
**作者**: Smith, J.R. et al.
**摘要**: 本研究报道了大肠杆菌中nadB基因的克隆与重组表达，通过优化表达条件获得高活性L-天冬氨酸氧化酶，并验证其在NAD生物合成途径中的功能，为体外酶催化研究提供基础。
2. **文献名称**: *Structural Insights into nadB-Encoded Enzyme: Crystallographic Analysis of Recombinant L-Aspartate Oxidase*
**作者**: Chen, L. & Wang, H.
**摘要**: 通过X射线晶体学解析了重组nadB蛋白的三维结构，揭示了其底物结合位点及催化机制，为设计靶向抑制剂或代谢工程改造提供结构基础。
3. **文献名称**: *Metabolic Engineering of Bacillus subtilis Using Recombinant nadB for Enhanced NADPH Production*
**作者**: Lee, S. et al.
**摘要**: 利用重组nadB基因在枯草芽孢杆菌中过表达L-天冬氨酸氧化酶，显著提高了NADPH的细胞内水平，优化了工业菌株的辅酶平衡及代谢产物合成效率。
4. **文献名称**: *Heterologous Expression of nadB in Yeast: Implications for NAD Biosynthesis and Cellular Redox Homeostasis*
**作者**: González, A. et al.
**摘要**: 在酿酒酵母中异源表达细菌来源的nadB基因，探究其对酵母NAD合成途径的补充作用及对氧化应激耐受性的影响，为真核生物代谢工程提供新策略。
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以上文献摘要概括了nadB重组蛋白在基因功能解析、结构研究、代谢工程及跨物种应用等领域的研究方向。如需真实文献，建议通过PubMed或Web of Science以“nadB recombinant protein”为关键词检索。
**Background of NadB Recombinant Protein**
The *nadB* gene encodes L-aspartate oxidase, a key enzyme in the *de novo* biosynthesis pathway of nicotinamide adenine dinucleotide (NAD), an essential coenzyme involved in redox reactions and cellular metabolism across all domains of life. In bacteria, NadB catalyzes the oxidation of L-aspartate to iminoaspartate, the first committed step in the production of quinolinic acid, a precursor of NAD. This pathway is absent in humans, making NadB a potential target for antimicrobial drug development.
Recombinant NadB protein is produced by cloning the *nadB* gene into expression vectors (e.g., *E. coli*) and purifying the protein for functional and structural studies. Its recombinant form enables high-yield production, facilitating research on enzyme kinetics, substrate specificity, and inhibitor screening. Structural analyses, including X-ray crystallography, have revealed details about its active site and regulatory mechanisms, such as feedback inhibition by NAD.
NadB's role in NAD biosynthesis has implications in metabolic engineering. Modulating its activity can optimize NAD(H) pools in microbial cell factories, enhancing the production of biofuels, pharmaceuticals, or nutraceuticals. Additionally, studies on NadB homologs in pathogens like *Helicobacter pylori* or *Pseudomonas aeruginosa* support efforts to design species-specific inhibitors, leveraging its absence in human metabolism to reduce off-target effects.
Recent advances in synthetic biology and protein engineering have further explored NadB variants with improved stability or altered regulation, broadening its applications in industrial biotechnology and therapeutics. Understanding NadB's function and regulation thus bridges fundamental biochemistry with practical innovations in health and sustainability.
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