| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | pac |
| Uniprot No | P13249 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA |
| 预测分子量 | 37.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAC重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
1. **文献名称**: *Production and Characterization of PAC Recombinant Protein for Targeted Cancer Therapy*
**作者**: Zhang L, et al.
**摘要**: 研究报道了在哺乳动物细胞系统中高效表达PAC重组蛋白的方法，验证其与肿瘤细胞表面受体特异性结合的能力，并展示其在靶向药物递送中的潜在应用。
2. **文献名称**: *PAC Recombinant Protein Enhances Neuronal Regeneration in Vitro*
**作者**: Patel S, et al.
**摘要**: 实验证明PAC重组蛋白通过激活PI3K/Akt信号通路促进神经元轴突再生，为神经退行性疾病的治疗提供了新的分子工具。
3. **文献名称**: *Structural and Functional Insights into PAC Recombinant Protein via Cryo-EM Analysis*
**作者**: Kim T, et al.
**摘要**: 利用冷冻电镜解析PAC重组蛋白的三维结构，揭示其与配体相互作用的分子机制，为基于结构的药物设计奠定基础。
(注：以上文献为示例，非真实存在。实际研究中建议通过PubMed或Web of Science检索具体文献。)
**Background of PAC Recombinant Proteins**
Recombinant proteins, engineered through genetic modification, are pivotal tools in biomedical research and therapeutic development. PAC (Puromycin-Acid Ceramidase) recombinant proteins represent a specialized class designed to combine functional domains for targeted applications. The PAC system typically integrates puromycin, an antibiotic that inhibits protein synthesis by ribosome binding, with acid ceramidase, an enzyme regulating ceramide metabolism. This fusion enables dual functionality: puromycin serves as a selection marker for transfected cells, while acid ceramidase modulates cellular lipid signaling, influencing processes like apoptosis and inflammation.
The development of PAC recombinant proteins leverages recombinant DNA technology, where genes encoding these domains are cloned into expression vectors and expressed in host systems (e.g., *E. coli* or mammalian cells). Post-translationally, the protein is purified via affinity tags, ensuring high specificity and activity. PAC variants are often tailored for studies in cancer, neurodegenerative diseases, or metabolic disorders, where ceramide pathways play critical roles.
Applications include *in vitro* cell line selection, gene-editing validation, and disease modeling. For instance, PAC-expressing vectors enable rapid isolation of genetically modified cells under puromycin pressure, streamlining experimental workflows. Additionally, acid ceramidase activity can be assayed to study lipid-mediated cellular responses or screen therapeutic candidates.
PAC recombinant proteins exemplify the convergence of selection efficiency and functional versatility, addressing challenges in translational research. Their design underscores the adaptability of recombinant protein engineering to meet evolving demands in drug discovery and mechanistic studies.
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