| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | rsrIM |
| Uniprot No | P14751 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319aa |
| 氨基酸序列 | MANRSHHNAGHRAMNALRKSGQKHSSESQLGSSEIGTTRHVYDVCDCLDTLAKLPDDSVQLIICDPPYNIMLADWDDHMDYIGWAKRWLAEAERVLSPTGSIAIFGGLQYQGEAGSGDLISIISHMRQNSKMLLANLIIWNYPNGMSAQRFFANRHEEIAWFAKTKKYFFDLDAVREPYDEETKAAYMKDKRLNPESVEKGRNPTNVWRMSRLNGNSLERVGHPTQKPAAVIERLVRALSHPGSTVLDFFAGSGVTARVAIQEGRNSICTDAAPVFKEYYQKQLTFLQDDGLIDKARSYEIVEGAANFGAALQRGDVAS |
| 预测分子量 | 51.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rsrIM重组蛋白的参考文献示例(内容基于假设性文献整理，供参考)：
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1. **文献名称**：*Cloning and Functional Characterization of the rsrIM Methyltransferase from Escherichia coli*
**作者**：Smith, J. et al.
**摘要**：本研究报道了rsrIM基因的克隆及其在大肠杆菌中的重组表达，证实其编码的蛋白具有DNA甲基转移酶活性，特异性地甲基化特定序列位点，参与细菌限制-修饰系统的自我保护机制。
2. **文献名称**：*Structural Insights into rsrIM Recombinant Protein through Crystallographic Analysis*
**作者**：Zhang, Y. et al.
**摘要**：通过X射线晶体学解析了重组rsrIM蛋白的三维结构，揭示了其底物结合域和催化中心的构象，为阐明其甲基化机制及设计抑制剂提供了结构基础。
3. **文献名称**：*Optimization of rsrIM Expression and Purification for High-Throughput Methylation Assays*
**作者**：Lee, H. et al.
**摘要**：优化了rsrIM重组蛋白在大肠杆菌中的表达条件，建立高效镍柱亲和层析纯化流程，并验证其在体外甲基化反应中的稳定性与酶活性，适用于高通量筛选。
4. **文献名称**：*Role of rsrIM in Bacterial Epigenetic Regulation: A Recombinant Protein-Based Study*
**作者**：Patel, R. & Brown, K.
**摘要**：利用重组rsrIM蛋白探究其对宿主DNA甲基化模式的影响，发现其通过表观遗传调控参与细菌毒力基因表达，为病原菌适应性机制研究提供新视角。
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注：以上文献为模拟示例，实际研究中建议通过**PubMed**或**Web of Science**以关键词“rsrIM methyltransferase recombinant”检索最新文献。
**Background of rsrIM Recombinant Protein**
The rsrIM recombinant protein is a type II DNA methyltransferase (MTase) derived from the *Rhodococcus* sp. RSR2 bacterial strain. It is part of a restriction-modification (R-M) system, a defense mechanism used by bacteria to protect their genome from foreign DNA, such as bacteriophages. The rsrIM enzyme specifically recognizes the DNA sequence 5'-GAATTC-3' (the same site as EcoRI) and methylates the adenine residue within this site, converting it to N6-methyladenine. This methylation prevents cleavage by the cognate restriction enzyme (RsrI), thereby safeguarding the host DNA while leaving unmethylated foreign DNA vulnerable to degradation.
Recombinant rsrIM is produced through genetic engineering, typically by cloning the rsrIM gene into expression vectors (e.g., in *E. coli*), followed by purification to homogeneity. Its ability to site-specifically modify DNA makes it a valuable tool in molecular biology for applications such as DNA methylation studies, epigenetic research, and synthetic biology. For instance, it is used to protect plasmids during cloning, generate methylated DNA standards, or study the role of methylation in gene regulation.
Structurally, rsrIM contains conserved MTase motifs, including the AdoMet-binding domain and catalytic sites essential for transferring methyl groups from S-adenosylmethionine (SAM) to DNA. Its recombinant form offers advantages over native proteins, such as higher purity, scalability, and reduced batch variability. Additionally, its compatibility with common laboratory workflows has solidified its role in genome engineering and CRISPR-based technologies, where precise methylation control is critical.
Overall, rsrIM exemplifies how bacterial defense systems can be repurposed as versatile biotechnological tools, bridging fundamental research and industrial applications in genetic manipulation and epigenomics.
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