| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMP2 |
| Uniprot No | P02689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MSNKFLGTWKLVSSENFDDYMKALGVGLATRKLGNLAKPTVIISKKGDIITIRTESTFKNTEISFKLGQEFEETTADNRKTKSIVTLQRGSLNQVQRWDGKETTIKRKLVNGKMVAECKMKGVVCTRIYEKV |
| 预测分子量 | 41.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMP2重组蛋白的3篇参考文献示例(注：文献为虚构示例，实际文献需通过学术数据库检索)：
1. **文献名称**：*Recombinant PMP2 Expression and Its Role in Lipid Membrane Assembly*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统表达重组PMP2蛋白，证实其与脂质膜结合的能力，并揭示其在髓鞘形成中的潜在作用。
2. **文献名称**：*Structural Characterization of Recombinant Human PMP2 Using X-ray Crystallography*
**作者**：Lee H, et al.
**摘要**：解析重组人源PMP2的晶体结构，阐明其脂质结合域的关键氨基酸残基，为周围神经病变机制提供结构依据。
3. **文献名称**：*Therapeutic Potential of Recombinant PMP2 in a Mouse Model of Charcot-Marie-Tooth Disease*
**作者**：Garcia R, et al.
**摘要**：在小鼠模型中验证重组PMP2蛋白的神经保护功能，证明其可改善髓鞘缺陷并缓解运动功能障碍。
建议通过PubMed或Google Scholar以关键词“PMP2 recombinant protein”、“Peripheral Myelin Protein 2 expression”检索真实文献。
PMP2 (Peripheral Myelin Protein 2), also known as fatty acid-binding protein 8 (FABP8), is a small, structurally conserved protein predominantly expressed in Schwann cells of the peripheral nervous system. It plays a critical role in maintaining myelin integrity by facilitating lipid transport and membrane organization. PMP2 binds long-chain fatty acids and cholesterol, contributing to the compaction and stability of myelin sheaths that insulate axons, ensuring efficient nerve signal conduction. Dysregulation of PMP2 has been linked to peripheral neuropathies, including Charcot-Marie-Tooth disease, highlighting its importance in neural health.
Recombinant PMP2 protein is engineered using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to produce high-purity, functional protein for research and therapeutic applications. Its recombinant form retains the native structure and lipid-binding capacity, enabling studies on myelin assembly, lipid metabolism, and disease mechanisms. Researchers utilize PMP2 recombinant protein to investigate molecular interactions in demyelinating disorders, screen potential drug candidates, and develop diagnostic tools. Additionally, it serves as a critical reagent for generating antibodies or validating genetic mutations in clinical settings.
The development of recombinant PMP2 addresses challenges in sourcing native protein from biological tissues, ensuring reproducibility and scalability. Its applications extend to understanding neurodevelopmental processes, toxicology assessments, and exploring therapeutic strategies for peripheral nerve regeneration. As a model protein in structural biology, PMP2 also aids in elucidating fatty acid-binding protein family dynamics, offering insights into broader cellular lipid homeostasis. Overall, recombinant PMP2 represents a vital tool bridging basic neuroscience research and translational medicine.
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