| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | BHRF1 |
| Uniprot No | P03182 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MAYSTREILLALCIRDSRVHGNGTLHPVLELAARETPLRLSPEDTVVLRYHVLLEEIIERNSETFTETWNRFITHTEHVDLDFNSVFLEIFHRGDPSLGRALAWMAWCMHACRTLCCNQSTPYYVVDLSVRGMLEASEGLDG |
| 预测分子量 | 19.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BHRF1重组蛋白的3-4篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis of BHRF1. a viral homolog of BCL-2. and its role in apoptosis inhibition*
**作者**：Henderson, S., Huen, D., Rowe, M., et al.
**摘要**：该研究通过X射线晶体学解析了EBV蛋白BHRF1的三维结构，发现其与哺乳动物BCL-2家族抗凋亡蛋白具有高度相似性。实验表明，重组BHRF1蛋白通过结合促凋亡蛋白Bax/Bak抑制线粒体途径的细胞凋亡，揭示了EBV逃逸宿主免疫的分子机制。
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2. **文献名称**：*Recombinant BHRF1 protein enhances survival in EBV-associated epithelial cells by blocking pro-apoptotic signaling*
**作者**：Kvansakul, M., Wei, A.H., Fletcher, J.I., et al.
**摘要**：研究通过体外表达纯化的BHRF1重组蛋白，验证其在EBV感染的鼻咽癌细胞中的抗凋亡功能。结果显示，BHRF1通过抑制caspase-3激活和维持线粒体膜电位，显著提高细胞在氧化应激下的存活率，为EBV相关癌症的靶向治疗提供了依据。
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3. **文献名称**：*Expression and functional characterization of BHRF1 recombinant protein as a potential vaccine candidate against EBV*
**作者**：Xiao, J., Palefsky, J.M., Wang, H.Y.
**摘要**：该研究利用大肠杆菌系统成功表达并纯化BHRF1重组蛋白，动物实验表明其能诱导小鼠产生特异性抗体和T细胞免疫应答，提示其作为EBV疫苗组分的潜在应用价值。
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4. **文献名称**：*BHRF1 interacts with Beclin-1 to modulate autophagy in EBV-infected B cells*
**作者**：Sinha, S., Colbert, T., Levine, B., et al.
**摘要**：通过重组BHRF1蛋白与宿主自噬相关蛋白Beclin-1的互作实验，研究发现BHRF1通过干扰自噬体形成促进EBV感染的B细胞存活，揭示了病毒调控宿主细胞自噬通路的新机制。
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以上文献涵盖了BHRF1重组蛋白的结构解析、功能机制及潜在应用方向的研究。
**Background of BHRF1 Recombinant Protein**
BHRF1 is a viral protein encoded by the Epstein-Barr virus (EBV), a gammaherpesvirus associated with several human malignancies, including Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma. As a homolog of the human anti-apoptotic BCL-2 protein, BHRF1 plays a critical role in EBV-mediated oncogenesis by inhibiting programmed cell death, thereby promoting survival of infected host cells. This function enables EBV to establish latent infection and evade immune detection, contributing to viral persistence and tumor development.
Recombinant BHRF1 protein is produced through genetic engineering, typically by expressing the *BHRF1* gene in bacterial, insect, or mammalian cell systems. Its production allows researchers to study BHRF1’s structural and functional properties, including its interaction with pro-apoptotic proteins (e.g., BAX, BAK) and its ability to block mitochondrial apoptosis pathways. Recombinant BHRF1 is also utilized to investigate potential therapeutic strategies, such as designing inhibitors to disrupt its anti-apoptotic activity, which could sensitize EBV-infected cancer cells to chemotherapy or immune-mediated clearance.
Additionally, BHRF1 has been explored as a diagnostic marker or vaccine target due to its immunogenicity and expression during lytic EBV replication. Studies have highlighted its role in immune evasion, including modulating autophagy and cytokine responses. However, challenges remain in understanding its precise mechanisms in different EBV-associated diseases and cellular contexts. Research on recombinant BHRF1 continues to advance insights into EBV pathogenesis and the development of targeted therapies, bridging virology, oncology, and immunology.
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