| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENTPD6 |
| Uniprot No | O75354 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 61-484aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSKWHRATATQAFFSITRAAPGARWGQQA HSPLGTAADGHEVFYGIMFDAGSTGTRVHVFQFTRPPRETPTLTHETFKA LKPGLSAYADDVEKSAQGIRELLDVAKQDIPFDFWKATPLVLKATAGLRL LPGEKAQKLLQKVKEVFKASPFLVGDDCVSIMNGTDEGVSAWITINFLTG SLKTPGGSSVGMLDLGGGSTQIAFLPRVEGTLQASPPGYLTALRMFNRTY KLYSYSYLGLGLMSARLAILGGVEGQPAKDGKELVSPCLSPSFKGEWEHA EVTYRVSGQKAAASLHELCAARVSEVLQNRVHRTEEVKHVDFYAFSYYYD LAAGVGLIDAEKGGSLVVGDFEIAAKYVCRTLETQPQSSPFSCMDLTYVS LLLQEFGFPRSKVLKLTRKIDNVETSWALGAIFHYIDSLNRQKSPAS |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENTPD6重组蛋白的3篇代表性文献的简要概括(注：以下为模拟示例，实际文献需通过学术数据库检索确认)：
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1. **标题**：Expression and Characterization of Recombinant Human ENTPD6 in Escherichia coli
**作者**：Smith J, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人源ENTPD6蛋白，证实其具有水解ATP和ADP的酶活性，并优化了其表达条件以增强稳定性。
2. **标题**：ENTPD6 Regulates Purinergic Signaling in Neuronal Cells via Extracellular Nucleotide Metabolism
**作者**：Lee H, et al.
**摘要**：利用重组ENTPD6蛋白进行体外实验，发现其通过降解细胞外ATP/ADP调控神经元突触传递，提示其在神经信号通路中的潜在作用。
3. **标题**：Structural Insights into ENTPD6 Catalytic Mechanism by X-ray Crystallography
**作者**：Wang Y, et al.
**摘要**：通过重组ENTPD6蛋白的晶体结构解析，揭示了其底物结合口袋的关键氨基酸残基，为开发靶向抑制剂提供了结构基础。
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如需具体文献，建议在PubMed或Web of Science中搜索关键词“ENTPD6 recombinant protein”获取最新研究。
ENTPD6 (Ectonucleoside Triphosphate Diphosphohydrolase 6), also known as CD39L2. is a member of the ectonucleoside triphosphate diphosphohydrolase family. This transmembrane glycoprotein hydrolyzes extracellular nucleoside triphosphates (NTPs) and diphosphates (NDPs) to monophosphates (NMPs), playing a regulatory role in purinergic signaling pathways. ENTPD6 is characterized by two transmembrane domains, a short intracellular N-terminus, and a large extracellular loop containing conserved catalytic apyrase regions with five apyrase-conserved motifs critical for enzymatic activity. Unlike other ENTPD family members (e.g., ENTPD1/CD39), ENTPD6 exhibits substrate specificity biased toward nucleoside diphosphates, particularly UDP and GDP, and functions optimally in alkaline pH conditions.
Physiologically, ENTPD6 is predominantly expressed in intracellular compartments, such as the Golgi apparatus and endoplasmic reticulum, suggesting a role in regulating luminal nucleotide concentrations. It influences glycosylation processes by modulating UDP levels, which are essential substrates for glycosyltransferases. Dysregulation of ENTPD6 has been implicated in metabolic disorders and cancer progression, where altered nucleotide metabolism may promote tumor cell survival or immune evasion.
Recombinant ENTPD6 protein is produced using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. It serves as a tool for studying enzyme kinetics, substrate specificity, and structure-function relationships. Therapeutic research explores its potential in targeting nucleotide-dependent pathways, including inflammation and glycosylation-related diseases. Recent studies also investigate its interaction with other ectonucleotidases and receptors in the purinergic signaling network, highlighting its nuanced role in cellular homeostasis.
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