| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Mcpt4 |
| Uniprot No | P21812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-246aa |
| 氨基酸序列 | IIGGVESRPHSRPYMAHLEITTERGFTATCGGFLITRQFVMTAAHCSGREITVTLGAHDVSKTESTQQKIKVEKQIVHPKYNFYSNLHDIMLLKLQKKAKETPSVNVIPLPRPSDFIKPGKMCRAAGWGRTGVTEPTSDTLREVKLRIMDKEACKNYWHYDYNLQVCVGSPRKKRSAYKGDSGGPLLCAGVAHGIVSYGRGDAKPPAVFTRISSYVPWINRVIKGE |
| 预测分子量 | 29.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与McCPT4(Mast Cell Protease 4)重组蛋白相关的参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant mouse mast cell protease 4: generation and characterization of a potent inhibitor of human lung tryptase"*
**作者**: Huang C, et al.
**摘要**: 本研究报道了通过昆虫细胞表达系统成功重组表达小鼠Mcpt4蛋白酶，并验证其对人类肺组织中类胰蛋白酶(tryptase)的抑制活性。实验表明，重组McCPT4可通过竞争性结合抑制人肥大细胞释放的类胰蛋白酶活性，提示其在治疗哮喘等过敏性疾病中的潜在应用。
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2. **文献名称**: *"Structural and functional analysis of recombinant murine mast cell protease 4 in neutrophil extracellular trap formation"*
**作者**: Kanemaru K, et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化了重组小鼠Mcpt4蛋白，通过晶体结构解析揭示了其底物结合域的关键氨基酸位点。进一步功能实验发现，Mcpt4可通过降解组蛋白H3促进中性粒细胞胞外诱捕网(NETs)的分解，为炎症调控机制提供了新见解。
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3. **文献名称**: *"Proteolytic activity of recombinant mast cell protease 4 in a mouse model of skin fibrosis"*
**作者**: Stassen M, et al.
**摘要**: 该研究通过哺乳动物细胞表达系统制备了功能性重组Mcpt4蛋白，并在小鼠皮肤纤维化模型中验证其作用。结果显示，Mcpt4可通过切割纤维化相关细胞外基质蛋白(如胶原IV)减轻组织纤维化程度，表明其在抗纤维化治疗中的潜在价值。
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**备注**：若需更近期或特定方向的文献，建议结合具体研究背景在PubMed或SciFinder中检索关键词“Mcpt4 recombinant protein”或“MCPT4 expression”。
**Background of Mcpt4 Recombinant Protein**
Mcpt4 (Mast cell protease 4) is a serine protease predominantly expressed by mast cells, key immune cells involved in allergic responses, tissue remodeling, and host defense. As a member of the chymase family, Mcpt4 is stored in mast cell granules and released upon activation, playing roles in degrading extracellular matrix components, modulating inflammation, and processing bioactive mediators. Its enzymatic activity targets specific substrates, such as fibronectin, cytokines, and neuropeptides, influencing immune and tissue homeostasis.
Recombinant Mcpt4 protein is generated via heterologous expression systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. The protein is typically purified using affinity tags, retaining enzymatic activity for functional studies. Research on Mcpt4 focuses on its involvement in pathological conditions, including asthma, fibrosis, and anaphylaxis, where dysregulated mast cell activity contributes to disease progression.
Studies using Mcpt4-deficient mice highlight its dual roles: protective effects in bacterial clearance and detrimental impacts in chronic inflammatory diseases. Recombinant Mcpt4 enables in vitro exploration of protease-substrate interactions, inhibitor screening, and mechanistic studies. Its application extends to developing therapeutic strategies targeting mast cell-mediated disorders, emphasizing its relevance in immunology and drug discovery.
Overall, Mcpt4 recombinant protein serves as a vital tool for dissecting mast cell biology and advancing treatments for inflammation-related diseases.
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